与蟹神经动作电位相关的光散射变化。
Changes in light scattering associated with the action potential in crab nerves.
作者信息
Cohen L B, Keynes R D
出版信息
J Physiol. 1971 Jan;212(1):259-75. doi: 10.1113/jphysiol.1971.sp009321.
Abstract
- Changes in light scattering from stimulated crab leg nerves were measured in an effort to study changes in structure that occur during the action potential.2. The peak changes in scattering after individual stimuli were 10(-4)-10(-5) of the resting scattering; signal averaging techniques were used to increase the signal-to-noise ratio.3. During the compound action potential there was a transient increase in the 90 degrees scattering which reached a peak about 20 msec after the stimulus. This increase was probably not due to a change in axon volume. Its time course was somewhat slower than that of birefringence changes measured at the same time.4. In normal sea water the transient increase was followed by a longer lasting (persistent) decrease in scattering. This persistent decrease could be converted to an increase by reducing the tonicity of the sea water or by raising the external refractive index. This scattering change may represent a swelling of the axon resulting from the ionic exchanges that occur during the action potential.
摘要
为了研究动作电位期间发生的结构变化，对受刺激的蟹腿神经的光散射变化进行了测量。
单个刺激后散射的峰值变化为静息散射的10（-4）-10（-5）；使用信号平均技术来提高信噪比。
在复合动作电位期间，90度散射有短暂增加，在刺激后约20毫秒达到峰值。这种增加可能不是由于轴突体积的变化。其时间进程比同时测量的双折射变化稍慢。
在正常海水中，短暂增加之后是散射的持续时间更长（持久）的减少。通过降低海水的张力或提高外部折射率，这种持久的减少可以转变为增加。这种散射变化可能代表动作电位期间发生的离子交换导致的轴突肿胀。
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