与蟹神经动作电位相关的光散射变化。
Changes in light scattering associated with the action potential in crab nerves.
作者信息
Cohen L B, Keynes R D
出版信息
J Physiol. 1971 Jan;212(1):259-75. doi: 10.1113/jphysiol.1971.sp009321.
Abstract
- Changes in light scattering from stimulated crab leg nerves were measured in an effort to study changes in structure that occur during the action potential.2. The peak changes in scattering after individual stimuli were 10(-4)-10(-5) of the resting scattering; signal averaging techniques were used to increase the signal-to-noise ratio.3. During the compound action potential there was a transient increase in the 90 degrees scattering which reached a peak about 20 msec after the stimulus. This increase was probably not due to a change in axon volume. Its time course was somewhat slower than that of birefringence changes measured at the same time.4. In normal sea water the transient increase was followed by a longer lasting (persistent) decrease in scattering. This persistent decrease could be converted to an increase by reducing the tonicity of the sea water or by raising the external refractive index. This scattering change may represent a swelling of the axon resulting from the ionic exchanges that occur during the action potential.
摘要
为了研究动作电位期间发生的结构变化，对受刺激的蟹腿神经的光散射变化进行了测量。
单个刺激后散射的峰值变化为静息散射的10（-4）-10（-5）；使用信号平均技术来提高信噪比。
在复合动作电位期间，90度散射有短暂增加，在刺激后约20毫秒达到峰值。这种增加可能不是由于轴突体积的变化。其时间进程比同时测量的双折射变化稍慢。
在正常海水中，短暂增加之后是散射的持续时间更长（持久）的减少。通过降低海水的张力或提高外部折射率，这种持久的减少可以转变为增加。这种散射变化可能代表动作电位期间发生的离子交换导致的轴突肿胀。
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本文引用的文献
1
The depolarization of crustacean nerve by stimulation or oxygen want.通过刺激或缺氧使甲壳类动物神经去极化。
J Physiol. 1929 Jul 25;67(4):325-42. doi: 10.1113/jphysiol.1929.sp002573.
2
Opacity changes in stimulated nerve.受刺激神经的透明度变化。
J Physiol. 1949 May 15;108(3):278-81.
3
The ionic movements during nervous activity.神经活动期间的离子运动。
J Physiol. 1951 Jun;114(1-2):119-50. doi: 10.1113/jphysiol.1951.sp004608.
4
The volume change resulting from stimulation of a giant nerve fibre.刺激一条巨大神经纤维所导致的体积变化。
J Physiol. 1950 Oct 16;111(3-4):304-27. doi: 10.1113/jphysiol.1950.sp004481.
5
The effect of stimulation on the opacity of a crustacean nerve trunk and its relation to fibre diameter.刺激对甲壳类动物神经干透明度的影响及其与纤维直径的关系。
J Physiol. 1950 Oct 16;111(3-4):283-303. doi: 10.1113/jphysiol.1950.sp004480.
6
The permeability of the squid giant axon to radioactive potassium and chloride ions.枪乌贼巨大轴突对放射性钾离子和氯离子的通透性。
J Physiol. 1960 Nov;154(1):177-89. doi: 10.1113/jphysiol.1960.sp006572.
7
The positive and negative heat production associated with a nerve impulse.与神经冲动相关的正热产生和负热产生。
Proc R Soc Lond B Biol Sci. 1958 Feb 18;148(931):149-87. doi: 10.1098/rspb.1958.0012.
8
Optical and mechanical concomitants of activity in Carcinus nerve. I. Effect of sodium azide on the optical response. II. Shortening of the nerve with activity.滨蟹神经活动的光学与机械伴随现象。I. 叠氮化钠对光学反应的影响。II. 神经活动时的缩短现象
J Cell Comp Physiol. 1955 Aug;46(1):71-95. doi: 10.1002/jcp.1030460105.
9
On the optical change associated with activity in frog nerve.关于与青蛙神经活动相关的光学变化。
J Cell Comp Physiol. 1955 Aug;46(1):53-70. doi: 10.1002/jcp.1030460104.
10
Some optically detectable consequences of activity in nerve.神经活动的一些可通过光学检测到的结果。
Cold Spring Harb Symp Quant Biol. 1952;17:15-25. doi: 10.1101/sqb.1952.017.01.004.
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