大鼠肝脏细胞外间隙和细胞内电解质的体内测定

Determination of extracellular space and intracellular electrolytes in rat liver in vivo.

Williams J A, Woodbury D M

J Physiol. 1971 Jan;212(1):85-99. doi: 10.1113/jphysiol.1971.sp009311.

Abstract

Uptake of a number of isotopically labelled extracellular tracers by rat liver in vivo was studied.2. A residual intravascular space of 3.0-3.5% following exsanguination was measured by use of [(51)Cr]red cells. This space was significantly greater than that of skeletal muscle and was also greater in nephrectomized as compared to intact rats.3. [(131)I]albumin and polyvinylpyrolidine (PVP) were found to distribute initially in a space of 6-7% and this was followed by a delayed rise that was greater for PVP. The extravascular portion of this space is termed the large-molecule accessible space and is believed to correspond to the histological space of Disse.4. [(14)C]sucrose and inulin initially distribute in a space of 10-12% followed by a continued late increase. This initial space is termed the small molecule accessible space and is thought to include most of interstitial space.5. (36)Cl(-) and stable Na(+) and Cl(-) spaces were constant at 19-21% and can be used to put an upper limit on extracellular space.6. Operationally, the 1 hr inulin space in the nephrectomized rat was felt to be the best indicator of extracellular space.7. Total electrolytes were measured and intracellular values of 22, 165 and 22 m-mole/l. cell water for Na(+), K(+) and Cl(-), respectively, were calculated.8. By use of [(14)C]DMO, an intracellular pH of 7.25 and an intracellular HCO(3) (-) of 13 m-mole/l. cell water were calculated.

摘要

研究了大鼠肝脏在体内对多种同位素标记的细胞外示踪剂的摄取情况。
放血后，利用[⁵¹Cr]红细胞测得残留血管空间为3.0 - 3.5%。该空间显著大于骨骼肌的残留血管空间，并且与完整大鼠相比，肾切除大鼠的该空间也更大。
发现[¹³¹I]白蛋白和聚乙烯吡咯烷酮（PVP）最初分布在6 - 7%的空间中，随后出现延迟上升，PVP的上升幅度更大。该空间的血管外部分称为大分子可及空间，被认为与狄氏间隙的组织学空间相对应。
[¹⁴C]蔗糖和菊粉最初分布在10 - 12%的空间中，随后持续缓慢增加。这个初始空间称为小分子可及空间，被认为包括大部分间质空间。
³⁶Cl⁻以及稳定的Na⁺和Cl⁻空间恒定在19 - 21%，可用于确定细胞外空间的上限。
从操作角度看，肾切除大鼠的1小时菊粉空间被认为是细胞外空间的最佳指标。
测量了总电解质，并计算出细胞内Na⁺、K⁺和Cl⁻的浓度分别为22、165和22毫摩尔/升细胞水。
利用[¹⁴C]二甲基氧（DMO），计算出细胞内pH值为7.25，细胞内HCO₃⁻为13毫摩尔/升细胞水。