人红细胞中胆碱转运所需的巯基（SH）基团的一些特性。
Some properties of an SH group essential for choline transport in human erythrocytes.
作者信息
Martin K
出版信息
J Physiol. 1971 Mar;213(3):647-64. doi: 10.1113/jphysiol.1971.sp009406.
Abstract
- The choline transport system in human erythrocytes is inhibited by N-ethylmaleimide (NEM), cystamine and p-chloromercuribenzene sulphonic acid (PCMBS).2. External choline increases the rate of inhibition by NEM and cystamine but decreases the rate of inhibition by PCMBS. Intracellular choline has the opposite effect.3. Competitive inhibitors of choline transport that are not themselves transported protect the carrier against all three thiol reagents.4. Some thiol reagents with a very low lipid solubility do not inhibit choline transport.5. The transport inhibition by cystamine is reversed by various reducing agents.6. Cystamine protects the transport system against NEM and PCMBS.7. It is suggested that NEM and cystamine react with an SH group of the transport system and that this SH group is more reactive when the carrier is facing inside. PCMBS penetrates erythrocytes only very slowly and is assumed to react preferentially with the outward facing carrier.8. The reactive SH group seems to be located in a lipophilic environment.
摘要
人红细胞中的胆碱转运系统受到N-乙基马来酰亚胺（NEM）、胱胺和对氯汞苯磺酸（PCMBS）的抑制。
细胞外胆碱会提高NEM和胱胺的抑制速率，但会降低PCMBS的抑制速率。细胞内胆碱则有相反的作用。
本身不被转运的胆碱转运竞争性抑制剂可保护载体免受所有三种硫醇试剂的影响。
一些脂溶性极低的硫醇试剂不会抑制胆碱转运。
胱胺引起的转运抑制可被各种还原剂逆转。
胱胺可保护转运系统免受NEM和PCMBS的影响。
有人提出，NEM和胱胺与转运系统的一个巯基反应，并且当载体面向细胞内部时，这个巯基更具反应性。PCMBS进入红细胞的速度非常缓慢，假定它优先与面向外部的载体反应。
反应性巯基似乎位于亲脂环境中。
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