红细胞的阳离子负载
Cation loading of red blood cells.
作者信息
Garrahan P J, Rega A F
出版信息
J Physiol. 1967 Nov;193(2):459-66. doi: 10.1113/jphysiol.1967.sp008371.
Abstract
- p-Chloromercuribenzenesulphonate induces an increase in the permeability of red cell membranes to monovalent cations. Under suitable conditions the increase in permeability is large enough to allow cell cation concentration to approach equilibrium with that in the suspending media in 24 hr at 5 degrees C.2. The effect of the mercurial on permeability is fully reversed by cysteine.3. These effects provide the basis for a simple procedure for preparing red blood cells of almost any desired sodium or potassium content.4. If hypertonic media are used, cells can be obtained with normal volume but in osmotic equilibrium with the hypertonic solutions.
摘要
对氯汞苯磺酸盐会导致红细胞膜对单价阳离子的通透性增加。在适宜条件下，通透性的增加幅度足以使细胞阳离子浓度在5摄氏度下24小时内接近与悬浮介质中的阳离子浓度达到平衡。
半胱氨酸可完全逆转汞剂对通透性的影响。
这些效应为制备几乎任何所需钠或钾含量的红细胞的简单程序提供了基础。
如果使用高渗介质，可以获得体积正常但与高渗溶液处于渗透平衡的细胞。
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本文引用的文献
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Cation exchanges of lactose-treated human red cells.乳糖处理的人红细胞的阳离子交换
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Interaction of mercury with human erythrocytes.汞与人体红细胞的相互作用。
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Uncoupling the sodium pump.使钠泵解偶联
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