Slapikoff S, Spitzer J L, Vaccaro D
J Bacteriol. 1971 Jun;106(3):739-44. doi: 10.1128/jb.106.3.739-744.1971.
Bacillus brevis ATCC 9999 sporulated without the production of extracellular protease when grown and allowed to sporulate on nutrient agar plates containing either azo-albumin, casein, or gelatin. Intracellular protease could not be detected in extracts of cells grown in nutrient broth-salts media. However, approximately one-fifth of the level of protease activity in extracts of B. subtilis SB 19 was found in extracts of B. brevis ATCC 9999 grown in minimal media. When protein turnover studies were performed by chasing previously incorporated (14)C-l-phenylalanine with a large excess of unlabeled phenylalanine, no protein turnover could be detected if the excess phenylalanine was added at T(0) and only a small amount of turnover (1 to 1.6% per hr) was detected if the excess phenylalanine was added at T(2.5-2.7). This compares with a measured turnover rate of 8.5% per hr in B. subtilis SB 19 under similar conditions.
短短芽孢杆菌ATCC 9999在含有偶氮白蛋白、酪蛋白或明胶的营养琼脂平板上生长并形成芽孢时，不会产生细胞外蛋白酶。在营养肉汤 - 盐培养基中生长的细胞提取物中未检测到细胞内蛋白酶。然而，在基本培养基中生长的短短芽孢杆菌ATCC 9999的提取物中，发现其蛋白酶活性水平约为枯草芽孢杆菌SB 19提取物中蛋白酶活性水平的五分之一。当通过用大量未标记的苯丙氨酸追踪先前掺入的（14）C - l - 苯丙氨酸进行蛋白质周转研究时，如果在T（0）时加入过量的苯丙氨酸，则检测不到蛋白质周转；如果在T（2.5 - 2.7）时加入过量的苯丙氨酸，则仅检测到少量周转（每小时1%至1.6%）。相比之下，在类似条件下，枯草芽孢杆菌SB 19的测得周转速率为每小时8.5%。
