Hageman J H, Carlton B C
J Bacteriol. 1973 May;114(2):612-7. doi: 10.1128/jb.114.2.612-617.1973.
Two protease-deficient mutants of Bacillus subtilis 168 (Trp(-)) were isolated and compared with the parental strain with respect to production of intracellular proteases and sporulation. A mutant lacking the metal-requiring "neutral" protease intracellularly sporulated as well as the parental strain. A second mutant, deficient in an as yet uncharacterized intracellular protease, failed to sporulate normally. It is proposed that this new protease is also involved in intracellular protein turnover.
分离出枯草芽孢杆菌168（色氨酸缺陷型）的两个蛋白酶缺陷型突变体，并就细胞内蛋白酶的产生和芽孢形成方面与亲本菌株进行了比较。一个细胞内缺乏需要金属的“中性”蛋白酶的突变体与亲本菌株一样形成芽孢。第二个突变体缺乏一种尚未鉴定的细胞内蛋白酶，不能正常形成芽孢。据推测，这种新的蛋白酶也参与细胞内蛋白质的周转。
