Biely P, Krátký Z, Kovarík J, Bauer S
J Bacteriol. 1971 Jul;107(1):121-9. doi: 10.1128/jb.107.1.121-129.1971.
The growth inhibition and the lysis of Saccharomyces cerevisiae caused by 2-deoxy-d-glucose (2-DG) were shown to be a consequence of unbalanced cellular growth and division. The lysis, but not the repression of growth and osmotic fragility of cells, could be suppressed by the addition of mannitol as an osmotic stabilizer. This result, as well as the morphological changes observed in the cells and changes in the chemical composition of the cell walls, showed that S. cerevisiae grown in the presence of 2-DG formed weakened cell walls responsible for the osmotic fragility. Evidence is presented for the first time demonstrating the incorporation of 2-DG into yeast cell wall material. Other data suggest that the inhibition of yeast growth by 2-DG results from an interference of phosphorylated metabolites of 2-DG with metabolic processes of glucose and mannose involved in the synthesis of structural cell wall polysaccharides.
2-脱氧-D-葡萄糖（2-DG）对酿酒酵母的生长抑制和裂解作用被证明是细胞生长和分裂失衡的结果。作为渗透稳定剂的甘露醇的添加可以抑制细胞的裂解，但不能抑制细胞生长的抑制和渗透脆性。这一结果，以及在细胞中观察到的形态变化和细胞壁化学成分的变化，表明在2-DG存在下生长的酿酒酵母形成了导致渗透脆性的弱化细胞壁。首次提供了证明2-DG掺入酵母细胞壁物质的证据。其他数据表明，2-DG对酵母生长的抑制是由于2-DG的磷酸化代谢产物干扰了参与结构性细胞壁多糖合成的葡萄糖和甘露糖的代谢过程。
