Donoghue D J
J Virol. 1982 May;42(2):538-46. doi: 10.1128/JVI.42.2.538-546.1982.
A clone of the Moloney murine sarcoma virus mos gene derived by in vitro reverse transcription was characterized. When assayed for focus formation by DNA transfection on NIH/3T3 cells, this clone was biologically inactive, presumably due to the absence of a long terminal repeat sequence. Therefore, a long terminal repeat was inserted into the clone by in vitro recombination, after which the most gene was able to transform NIH/3T3 cells efficiently. The nucleotide sequence encompassing the transforming region of this clone was determined. A single long open reading frame was observed, which potentially encodes a polypeptide of 41,000 daltons. This open reading frame initiates with the first five amino acids of the murine leukemia virus env gene, after which it enters the mos sequence, where it terminates. The nucleotide sequence described in this paper was compared with other sequences of mos in an effort to resolve discrepancies in the position of the long open reading frame. Although Moloney murine sarcoma virus retains the 3' splicing site of the murine leukemia virus env gene, a mos-specific mRNA which corresponds structurally to the murine leukemia virus env mRNA was not identified. The sequence described here revealed a single nucleotide change in the proposed env gene 3' splicing site which was retained in Moloney murine sarcoma virus. This deviation from the consensus 3' splicing sequence may underlie the observed absence of mos expression via the env gene splicing pathway.
对通过体外逆转录获得的莫洛尼鼠肉瘤病毒mos基因的一个克隆进行了表征。当通过DNA转染在NIH/3T3细胞上检测其形成集落的能力时，该克隆无生物学活性，推测是由于缺乏长末端重复序列。因此，通过体外重组将一个长末端重复序列插入该克隆中，之后mos基因能够有效地转化NIH/3T3细胞。确定了包含该克隆转化区域的核苷酸序列。观察到一个单一的长开放阅读框，它可能编码一个41,000道尔顿的多肽。这个开放阅读框从鼠白血病病毒env基因的前五个氨基酸开始，之后进入mos序列，并在那里终止。将本文所述的核苷酸序列与其他mos序列进行比较，以解决长开放阅读框位置上的差异。尽管莫洛尼鼠肉瘤病毒保留了鼠白血病病毒env基因的3'剪接位点，但未鉴定出在结构上与鼠白血病病毒env mRNA相对应的mos特异性mRNA。这里描述的序列揭示了在提议的env基因3'剪接位点上有一个单核苷酸变化，该变化在莫洛尼鼠肉瘤病毒中保留了下来。与共有3'剪接序列的这种偏差可能是通过env基因剪接途径观察到的mos表达缺失的原因。
