Messmer T O, Young D V
J Cell Physiol. 1977 Feb;90(2):265-7. doi: 10.1002/jcp.1040900212.
The growth of SV3T3 cells in medium containing a low concentration (0.20% v/v) of normal calf serum is enhanced by the addition of biotin or certain unsaturated fatty acids. The biotin effect on the final viable cell density is 5- to 10-fold over the control and is extremely potent, exerting a saturating response at a a concentration of approximately 200 pg/ml. The optimal growth response observed with fatty acids in 5-fold over the control and requires the combination of nervonic acid, palmitoleic acid, and arachidonic acid. The fatty acids are probably not replacing the function of biotin since these two substances are additive in their growth effects.
在含有低浓度（0.20% v/v）正常小牛血清的培养基中，添加生物素或某些不饱和脂肪酸可促进SV3T3细胞的生长。生物素对最终活细胞密度的影响比对照高5至10倍，且作用极强，在浓度约为200 pg/ml时产生饱和反应。观察到脂肪酸的最佳生长反应比对照高5倍，且需要神经酸、棕榈油酸和花生四烯酸共同作用。脂肪酸可能无法替代生物素的功能，因为这两种物质在生长效应上具有相加性。
