Underwood B O, Smale C J, Brown F, Hill B J
J Gen Virol. 1977 Jul;36(1):93-109. doi: 10.1099/0022-1317-36-1-93.
The physicochemical and serological properties of a virus isolated from the bivalve mollusc, Tellina tenuis, have been examined. The virus has a diam. of 59 nm, sediments at 430S in sucrose gradients and bands at a density of I-32 g/ml in CsCl. The virus contains RNA with a mol. wt. about 2-8 X 10(6) as extimated by polyacrylamide gel electrophoresis but in sucrose gradients the RNA sediments at 14S. The virus RNA is resistant to ribonuclease under conditions in which ribosomal RNA and the single stranded Mengo virus RNA are completely hydrolysed. Two major polypeptides, mol. wt. 67 and 40 X 10(3), and minor polypeptide, mol. wt. 110 X 10(3), are present in the virus particle. These properties are similar to those found in different serotypes of infectious pancreatic necrosis (IPN) virus. Although there was only a very low level of cross-neutralization between Tellina virus and IPN virus. there was some cross-reaction in immune electron microscopy tests and in immunofluorescence tests with infected tissue culture cells. This cross reaction, together with the close similarity in morphology and physiochemical properties, suggests that Tellina virus and IPN virus belong to the same virus group.
对从双壳贝类软体动物细纹蚬中分离出的一种病毒的理化和血清学特性进行了检测。该病毒直径为59纳米，在蔗糖梯度中于430S处沉降，在氯化铯中的密度为1.32克/毫升时形成条带。该病毒含有RNA，通过聚丙烯酰胺凝胶电泳估计其分子量约为2.8×10⁶，但在蔗糖梯度中RNA于14S处沉降。在核糖体RNA和单链门戈病毒RNA被完全水解的条件下，该病毒RNA对核糖核酸酶具有抗性。病毒粒子中存在两种主要多肽，分子量分别为67×10³和40×10³，以及一种次要多肽，分子量为110×10³。这些特性与在不同血清型的传染性胰腺坏死（IPN）病毒中发现的特性相似。尽管细纹蚬病毒与IPN病毒之间的交叉中和水平非常低，但在免疫电镜试验以及对感染的组织培养细胞进行的免疫荧光试验中存在一些交叉反应。这种交叉反应，连同形态和理化特性的密切相似性，表明细纹蚬病毒和IPN病毒属于同一病毒组。
