Makris A, Ryan K J
Steroids. 1977 Jan;29(1):65-72. doi: 10.1016/0039-128x(77)90109-x.
The major synthesis of estrogen by the follicle is postulated to require both theca and granulosa cells. Theca in this scheme provide androgens to the major aromatizing site in the follicle, and the granulosa cell. One aspect of this theory was tested here. We investigated the comparative ability of isolated granulosa and theca, alone and in recombination to aromatize androgen in vitro. We found that the granulosa aromatize [14C]substrate more efficiently than do theca, and compare with the recombined system in their ability to aromatize [14C]androgen. The data therefore substantiates one aspect of the theory regarding the nature of the synergism, i.e., that the granulosa cells, at least in vitro, are the major site of aromatization of the preovulatory follicle.
卵泡雌激素的主要合成过程据推测需要卵泡膜细胞和颗粒细胞共同参与。在这一机制中，卵泡膜细胞为卵泡内主要的芳香化位点即颗粒细胞提供雄激素。本研究对该理论的一个方面进行了验证。我们研究了分离的颗粒细胞和卵泡膜细胞单独以及重组后在体外将雄激素芳香化的能力。我们发现，颗粒细胞比卵泡膜细胞更有效地将[14C]底物芳香化，并且在将[14C]雄激素芳香化的能力方面与重组系统相当。因此，这些数据证实了关于协同作用本质的理论的一个方面，即至少在体外，颗粒细胞是排卵前卵泡芳香化的主要位点。
