Litwin J
Appl Microbiol. 1971 Apr;21(4):575-9. doi: 10.1128/am.21.4.575-579.1971.
The effect of varying centrifugal forces on the growth rate, longevity, and adsorption on glass of human embryonic diploid lung fibroblasts was studied. Cells centrifuged at 120, 500, or 1,500 x g at each passage had similar growth rates but their longevity decreased slightly with increasing force. These forces had no influence on the proportion of cells attaching to the glass. When the material in the first supernatant was recentrifuged at 2,000 x g for 30 min and added to the cells precipitated in the first centrifugation, the longevity of these cells was increased by several cell divisions. Cells which were not centrifuged but added directly from the cell suspension in trypsin to the new culture grew at a slightly slower rate than the centrifuged cells and became senescent at an earlier time. However, the noncentrifuged cells adsorbed to glass better than those centrifuged.
研究了不同离心力对人胚二倍体肺成纤维细胞生长速率、寿命及玻璃吸附的影响。每次传代时以120、500或1500×g离心的细胞具有相似的生长速率，但随着离心力增加其寿命略有下降。这些离心力对贴壁于玻璃的细胞比例无影响。当第一次上清液中的物质以2000×g再离心30分钟并加入第一次离心沉淀的细胞中时，这些细胞的寿命增加了几个细胞分裂周期。未离心而是直接从胰蛋白酶细胞悬液中加入新培养物的细胞生长速率略低于离心细胞，且更早衰老。然而，未离心的细胞比离心的细胞更易吸附于玻璃。
