Joyner A E, Winter W T, Godbout D M
Can J Microbiol. 1977 Mar;23(3):346-53. doi: 10.1139/m77-051.
Hydrogen production was studied in the following rumen anaerobes: Bacteroides clostridiiformis, Butyrivibrio fibrisolvens, Enbacterium limosum, Fusobacterium necrophorum, Megasphaera elsdenii, Ruminococcus albus, and Ruminococcus flavefaciens. Clostridium pasteurianum and Escherichia coli were included for comparative purposes. Hydrogen production from dithionite, dithionite-reduced methyl viologen, pyruvate, and formate was determined. All species tested produced hydrogen from dithionite-reduce methyl viologen, but only C. pasteurianum, B. clostridiiformis, E. limosum, and M. elsdenii produced hydrogen from dithionite. All species except E. coli produced hydrogen from pyruvate, but activity was low or absent in extracts of E. limosum, F. necrophorum, R. albus, and R. flavefaciens unless methyl viologen was added. Hydrogen was produced from formate only by E. coli, B. clostridiiformis, E. limosum, F. necrophorum, and R. flavefaciens. Extracts were subjected to ultracentrifugation in an effort to determine the solubility of hydrogenase. The hydrogenase of all species except E. coli appeared to be soluble, although variable amounts of hydrogenase activity were detected in the pellet. Treatment of extracts of the rumen microbial species with DEAE-cellulose resulted in loss ofhydrogen production from pyruvate. Activity was restored by the addition of methyl viologen. It is concluded that hydrogen production in these rumen microorganisms is similar to that in the saccharolytic clostridia.
梭状芽孢杆菌样拟杆菌、溶纤维丁酸弧菌、黏液真杆菌、坏死梭杆菌、埃氏巨球型菌、白色瘤胃球菌和黄色瘤胃球菌。为作比较，还纳入了巴氏梭菌和大肠杆菌。测定了连二亚硫酸盐、连二亚硫酸盐还原的甲基紫精、丙酮酸和甲酸的产氢情况。所有测试的菌种都能从连二亚硫酸盐还原的甲基紫精中产氢，但只有巴氏梭菌、梭状芽孢杆菌样拟杆菌、黏液真杆菌和埃氏巨球型菌能从连二亚硫酸盐中产氢。除大肠杆菌外的所有菌种都能从丙酮酸中产氢，但黏液真杆菌、坏死梭杆菌、白色瘤胃球菌和黄色瘤胃球菌的提取物中活性较低或无活性，除非添加甲基紫精。只有大肠杆菌、梭状芽孢杆菌样拟杆菌、黏液真杆菌、坏死梭杆菌和黄色瘤胃球菌能从甲酸中产氢。对提取物进行超速离心，以确定氢化酶的溶解性。除大肠杆菌外，所有菌种的氢化酶似乎都是可溶的，尽管在沉淀中检测到了不同量的氢化酶活性。用二乙氨基乙基纤维素处理瘤胃微生物菌种的提取物，导致丙酮酸产氢量减少。添加甲基紫精可恢复活性。得出的结论是，这些瘤胃微生物的产氢情况与解糖梭菌相似。
