Glass T L, Bryant M P, Wolin M J
J Bacteriol. 1977 Aug;131(2):463-72. doi: 10.1128/jb.131.2.463-472.1977.
Extracts of Ruminococcus albus were not able to convert pyruvate to acetyl phosphate, CO2, and H2 after passage through a diethylaminoethyl (DEAE)-cellulose column. Activity was restored by a brown protein fraction eluted from the column with 0.4 M Cl-. The protein was partially purified and shown to have the spectral and biological characteristics of ferredoxin. R. albus ferredoxin, Clostridium pasteurianum ferredoxin, and methyl viologen restored activity for pyruvate decomposition by DEAE-cellulose-treated R. albus extracts. R. albus or C. pasteurianum ferredoxin restored the ability of DEAE-cellulose-treated C. pasteurianum extracts to form H2 and acetyl phosphate from pyruvate. Ferredoxin-free extracts of R. albus reduced nicotinamide adenine dinucleotide (NAD) when supplemented with R. albus or C. pasteurianum ferredoxin or with methyl viologen. These extracts reduced NADP with H2 poorly unless both ferredoxin and NAD were added, which indicates the presence of an NADH:NADP transhydrogenase. Flavin mononucleotide and flavin adenine dinucleotide were rapidly reduced by H2 by ferredoxin-free extracts in the absence of ferredoxin.
经过二乙氨基乙基（DEAE）纤维素柱后，白色瘤胃球菌提取物无法将丙酮酸转化为乙酰磷酸、二氧化碳和氢气。用0.4M Cl-从柱上洗脱的棕色蛋白质组分可恢复活性。该蛋白质经过部分纯化，显示出具有铁氧化还原蛋白的光谱和生物学特性。白色瘤胃球菌铁氧化还原蛋白、巴氏梭菌铁氧化还原蛋白和甲基紫精可恢复经DEAE纤维素处理的白色瘤胃球菌提取物对丙酮酸分解的活性。白色瘤胃球菌或巴氏梭菌铁氧化还原蛋白可恢复经DEAE纤维素处理的巴氏梭菌提取物从丙酮酸形成氢气和乙酰磷酸的能力。当补充白色瘤胃球菌或巴氏梭菌铁氧化还原蛋白或甲基紫精时，白色瘤胃球菌不含铁氧化还原蛋白的提取物可还原烟酰胺腺嘌呤二核苷酸（NAD）。除非同时添加铁氧化还原蛋白和NAD，否则这些提取物用氢气还原NADP的能力很差，这表明存在NADH:NADP转氢酶。在没有铁氧化还原蛋白的情况下，不含铁氧化还原蛋白的提取物可通过氢气迅速还原黄素单核苷酸和黄素腺嘌呤二核苷酸。
