Wolin M J, Miller T L
Arch Microbiol. 1980 Feb;124(2-3):137-42. doi: 10.1007/BF00427718.
H2 production from glucose by Ruminococcus albus was almost completely inhibited by 10(-5) M molybdate only when sulfide was present in the growth medium. Inhibition was accompanied by a significant increase in the production of formate. Extracts of molybdate-sulfide-grown cells did not contain hydrogenase activity. Active enzyme in extracts of uninhibited cells was not inhibited by the molybdate-sulfide-containing growth medium. The results indicate that a complex formed from molybdate and sulfide prevents the formation of active hydrogenase and electrons otherwise used to form H2 are used to reduce CO2 to formate. Growth was significantly inhibited when molybdate was increased to 10(-4) M. Reversal of growth inhibition but not inhibition of H2 production occurred between 10(-4) and 10(-3) M molybdate. H2 production by R. bromei but not by R. flavefaciens, Butyrivibrio fibrisolvens, Veillonella alcalescens, Klebsiella pneumoniae and Escherichia coli was inhibited by molybdate and sulfide.
仅当生长培养基中存在硫化物时，10⁻⁵ M的钼酸盐几乎能完全抑制白色瘤胃球菌由葡萄糖产生氢气的过程。抑制作用伴随着甲酸盐产量的显著增加。经钼酸盐 - 硫化物培养的细胞提取物不含有氢化酶活性。未受抑制的细胞提取物中的活性酶不受含钼酸盐 - 硫化物的生长培养基抑制。结果表明，由钼酸盐和硫化物形成的复合物会阻止活性氢化酶的形成，否则用于形成氢气的电子会用于将二氧化碳还原为甲酸盐。当钼酸盐浓度增加到10⁻⁴ M时，生长受到显著抑制。在10⁻⁴至10⁻³ M的钼酸盐之间，生长抑制得以逆转，但氢气产生的抑制并未逆转。钼酸盐和硫化物会抑制布氏瘤胃球菌产生氢气，但不会抑制黄化瘤胃球菌、溶纤维丁酸弧菌、产碱韦荣球菌、肺炎克雷伯菌和大肠杆菌产生氢气。
