来自盘基网柄菌的多种形式的葡萄糖激酶。
Multiple forms of glucokinase from Dictyostelium discoideum.
作者信息
Killick K A, Wright B E
出版信息
J Bacteriol. 1978 Feb;133(2):1039-41. doi: 10.1128/jb.133.2.1039-1041.1978.
Abstract
A single form of glucokinase with an apparent Km value equal to 0.12 mM glucose was detectable in extracts prepared from aggregating cells, whereas kinetic and electrophoretic evidence indicated the presence of this form as well as a second glucose-phosphorylating enzyme with a Km value of about 0.01 mM glucose in extracts from culminating cells.
摘要
在从聚集细胞制备的提取物中可检测到一种表观Km值等于0.12 mM葡萄糖的单一形式的葡萄糖激酶，而动力学和电泳证据表明，在终末细胞的提取物中存在这种形式以及第二种葡萄糖磷酸化酶，其Km值约为0.01 mM葡萄糖。
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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