一种用于检测1,25-二羟维生素D3及其他抗佝偻病药物的体外生物测定法。
An in vitro bioassay for 1,25-dihydroxyvitamin D3 and other antirachitic agents.
作者信息
Parkes C O, Reynolds J J
出版信息
Mol Cell Endocrinol. 1977 Mar;7(1):25-31. doi: 10.1016/0303-7207(77)90073-9.
Duodenal tissue from chicken embryos (19 days in ovo) has been maintained in organ culture for 48 hours. The incubation medium and culture conditions were chosen to produce a linear relationship between calcium binding protein production and the logarithm of the dose of vitamin D3 steroid in the medium. The dose-response curves are parallel for vitamin D3 and both its major metabolites, 25-hydroxyvitamin D3 and 1,25-dihydroxyVITAMIN D3. The minimum concentration of 1,25-dihydroxyvitamin D3 capable of inducing calcium-binding-protein synthesis was 1 X 10(-9) M.
鸡胚（孵化19天）的十二指肠组织已在器官培养中维持48小时。选择孵育培养基和培养条件，以使钙结合蛋白的产生与培养基中维生素D3类固醇剂量的对数之间呈现线性关系。维生素D3及其两种主要代谢产物25-羟基维生素D3和1,25-二羟基维生素D3的剂量反应曲线是平行的。能够诱导钙结合蛋白合成的1,25-二羟基维生素D3的最低浓度为1×10^(-9)M。
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