Seidl S
Infusionsther Klin Ernahr. 1977 Apr;4(2):88-91.
For the past ten years we have been evaluating the preservation of human red cells by freezing. A comparative study of several techniques revealed no major differences as far as clinical effectiveness is concerned. The recovery percentage varies from 90-95%. Due to the washing procedure, necessary for removing the intracellular cryophylactic substance glycerol, no side effects from these ghosts have been observed. The maximum storage time in the post-thaw period should not exceed 24 hours, because the so-called "closed system" is interrupted. Since the preparations and storage of these cells is laborious and expensive, frozen blood should be used in selected clinical situations, i.e. rare blood types, autologous transfusions.
在过去十年里，我们一直在评估通过冷冻保存人类红细胞的方法。几项技术的对比研究表明，就临床效果而言，并无重大差异。恢复率在90%至95%之间。由于去除细胞内防冻物质甘油所需的洗涤程序，尚未观察到这些“空壳”产生任何副作用。解冻后阶段的最长储存时间不应超过24小时，因为所谓的“封闭系统”被中断了。由于这些细胞的制备和储存既费力又昂贵，冷冻血液应在特定临床情况下使用，即稀有血型、自体输血。
