Dawson G, Sundarraj N, Pfeiffer S E
J Biol Chem. 1977 Apr 25;252(8):2777-9.
Clonal cell lines derived from both spontaneous and chemically induced rat and mouse brain tumors were screened for their ability to incorporate H232SO4 into galactosyl(3-O-sulfate)ceramide (sulfatide). High levels of 35SO4 incorporation into sulfatide were found only in two of the mouse cell lines studied (G26-20 and -24). Tumors produced by subcutaneous injection of these cell lines into C57BL/6 mice were also unique in that they contained high levels of both sulfatide and galactosylceramide. The synthesis of large amounts of sulfatide and galactosylceramide by a clonal cell line of neurological origin suggests that the original tumor was of oligodendrocyte or Schwann cell origin. In common with a large number of mouse and rat astrocyte cell strains and their derived tumors, these glial cells lacked the ability to synthesize gangliosides such as monosialotetraglycosylceramide and disialotetraglycosylceramide (as judged by analytical and [3H]GlcNH2 incorporation studies). This appears to be a unique characteristic of neuroblastoma-derived cell strains such as N18, NB2a, and NB41A.
对源自自发和化学诱导的大鼠及小鼠脑肿瘤的克隆细胞系进行筛选，检测其将H232SO4掺入半乳糖基（3 - O - 硫酸酯）神经酰胺（硫脂）的能力。仅在研究的两个小鼠细胞系（G26 - 20和 - 24）中发现高水平的35SO4掺入硫脂。将这些细胞系皮下注射到C57BL / 6小鼠中产生的肿瘤也很独特，因为它们含有高水平的硫脂和半乳糖基神经酰胺。神经起源的克隆细胞系合成大量硫脂和半乳糖基神经酰胺，这表明原始肿瘤起源于少突胶质细胞或雪旺细胞。与大量小鼠和大鼠星形胶质细胞系及其衍生肿瘤一样，这些神经胶质细胞缺乏合成神经节苷脂的能力，如单唾液酸四糖基神经酰胺和双唾液酸四糖基神经酰胺（通过分析和[3H]GlcNH2掺入研究判断）。这似乎是神经母细胞瘤衍生细胞系（如N18、NB2a和NB41A）的独特特征。
