柠檬酸盐与碳水化合物向脂肪的转化。大鼠肝脏提取物对脂肪酸合成的调节。
Citrate and the conversion of carbohydrate into fat. The regulation of fatty acid synthesis by rat liver extracts.
作者信息
Fang M, Lowenstein J M
出版信息
Biochem J. 1967 Nov;105(2):803-11. doi: 10.1042/bj1050803.
Abstract
- The rate of fatty acid synthesis by particle-free extracts prepared from rat liver is increased greatly if the enzyme system is first activated with citrate. 2. The extent of the activation depends on the citrate concentration and on the time of activation in an interdependent manner. 3. Citrate activation is strongly dependent on temperature. 4. Tricarballylate can replace citrate as an activator, but its presence in the assay inhibits fatty acid synthesis. 5. Mg(2+) ions can replace citrate in the activation but not in the complete reaction system. 6. ATP prevents the activating effect of citrate and Mg(2+) ions. 7. The rate of fatty acid synthesis is increased by palmitoyl-dl-carnitine. This type of activation, additional to that caused by citrate, is rapid and does not depend on prior incubation. 8. Inhibition of fatty acid synthesis by palmitoyl-CoA can be prevented by palmitoyl-dl-carnitine or by increasing the concentration of protein.
摘要
- 如果先用柠檬酸盐激活酶系统，那么由大鼠肝脏制备的无颗粒提取物合成脂肪酸的速率会大幅提高。2. 激活程度以相互依存的方式取决于柠檬酸盐浓度和激活时间。3. 柠檬酸盐激活强烈依赖于温度。4. 三羧甲基丙烷可以替代柠檬酸盐作为激活剂，但它在测定体系中的存在会抑制脂肪酸合成。5. Mg(2+)离子可以在激活过程中替代柠檬酸盐，但在完整反应体系中不行。6. ATP会阻止柠檬酸盐和Mg(2+)离子的激活作用。7. 棕榈酰-dl-肉碱可提高脂肪酸合成速率。这种激活类型，除了由柠檬酸盐引起的激活外，速度很快且不依赖于预先孵育。8. 棕榈酰-CoA对脂肪酸合成的抑制作用可通过棕榈酰-dl-肉碱或增加蛋白质浓度来防止。
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CITRATE AND THE CONVERSION OF CARBOHYDRATE INTO FAT. THE ACTIVITIES OF CITRATE-CLEAVAGE ENZYME AND ACETATE THIOKINASE IN LIVERS OF STARVED AND RE-FED RATS.柠檬酸盐与碳水化合物向脂肪的转化。饥饿和重新喂食大鼠肝脏中柠檬酸裂解酶和乙酸硫激酶的活性。
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