三碘甲状腺原氨酸对培养的鸡胚心脏细胞摄取2-脱氧-D-（1-³H）葡萄糖的影响。
The effect of triiodothyronine on 2-deoxy-D-(1-3H)glucose uptake in cultured chick embryo heart cells.
作者信息
Segal J, Schwartz H, Gordon A
出版信息
Endocrinology. 1977 Jul;101(1):143-9. doi: 10.1210/endo-101-1-143.
The uptake of 2-deoxy-D-[1-3H]glucose ([1-3H]DOG)by cultured chick embryo heart cells was stimulated by exposure to triiodothyronine (T3). The concentration of free T3 for half maximal stimulation of [1-3H]DOG uptake was 8.7 x 10(-10)M. Nuclear binding studies revealed that the Kd for T3 was 4.2 x 10(-10)M. The molar potencies of other thyroactive compounds in stimulating [1-3H]DOG uptake, relative to L-T3, were: 500% for 3'-isopropyl-3,5-diiodo-L-thyronine, 20% for L-thyroxine (T4), 17% for D-T3, 2.2% for D-T4, 0.6% for 3,5-diiodo-L-thyronine and less than 0.1% for 3',5',3-triiodo-L-thyronine and L-thyronine. These data suggest that cultured chick embryo heart cells may serve in the study of the mode of action of thyroid hormones.
培养的鸡胚心脏细胞对2-脱氧-D-[1-³H]葡萄糖（[1-³H]DOG）的摄取受到三碘甲状腺原氨酸（T3）的刺激。对[1-³H]DOG摄取产生半数最大刺激作用时的游离T3浓度为8.7×10⁻¹⁰M。细胞核结合研究表明，T3的解离常数（Kd）为4.2×10⁻¹⁰M。相对于L-T3，其他甲状腺活性化合物在刺激[1-³H]DOG摄取方面的摩尔效力分别为：3'-异丙基-3,5-二碘-L-甲状腺原氨酸为500%，L-甲状腺素（T4）为20%，D-T3为17%，D-T4为2.2%，3,5-二碘-L-甲状腺原氨酸为0.6%，3',5',3-三碘-L-甲状腺原氨酸和L-甲状腺原氨酸小于0.1%。这些数据表明，培养的鸡胚心脏细胞可用于研究甲状腺激素的作用方式。
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