化学致癌物和诱变剂对BHK 21/13细胞的转化作用。
Transformation of BHK 21/13 cells by chemical carcinogens and mutagens.
作者信息
Altanerová V
出版信息
Neoplasma. 1975;22(6):599-606.
PMID:55982
Abstract
In vitro transformation of BHK 21/13 cells by chemical carcinogens is reported. The hamster cells were treated with MCA, BP, 4-NQO and 5-azaCR at various concentration and duration of treatment. The morphological, karyological and growth characteristics of cell lines were investigated. Search for RNA tumor viruses in transformed cells using 3H-uridine labeling, detection of DNA polymerases, electron microscopy investigations failed to detect presence of C-type virus particles.
摘要
据报道，化学致癌物可使BHK 21/13细胞发生体外转化。用不同浓度和处理时间的甲基胆蒽（MCA）、苯并芘（BP）、4-硝基喹啉-1-氧化物（4-NQO）和5-氮杂胞苷（5-azaCR）处理仓鼠细胞。研究了细胞系的形态学、染色体学和生长特性。利用3H-尿苷标记在转化细胞中寻找RNA肿瘤病毒、检测DNA聚合酶以及进行电子显微镜检查，均未检测到C型病毒颗粒的存在。
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