Black B L, Moog F
Science. 1977 Jul 22;197(4301):368-70. doi: 10.1126/science.560059.
If duodenal tissue from 14-day chick embryos is cultured in chemically defined medium, more than twice as many goblet cells appear within 48 hours as in intact embryos during the same time. The increase in goblet cell number is further accelerated by 10(-9) molar thyroxine but is prevented by 10(-6) molar hydrocortisone. The results suggest that differentiation of intestinal epithelium is regulated in part by a circulating inhibitor.
如果将14日龄鸡胚的十二指肠组织在化学成分明确的培养基中培养，48小时内出现的杯状细胞数量是同一时期完整胚胎中的两倍多。10(-9)摩尔的甲状腺素可进一步加速杯状细胞数量的增加，但10(-6)摩尔的氢化可的松可抑制这一增加。结果表明，肠上皮的分化部分受循环抑制剂的调节。
