Allan I J, Newgreen D F
Am J Anat. 1977 Jul;149(3):413-21. doi: 10.1002/aja.1001490306.
Catecholamine accumulation in chick embryos of stages 16 to 24 was investigated using formaldehyde-induced fluorescence. Fluorescence first appeared at stage 21 in the anterior sympathetic chain. After L-DOPA treatment, this fluorescence appeared at stage 18. Noradrenaline could not advance the onset of fluorescence or reconstitute fluorescence after its depletion by reserpine at stages 22 to 24. Under no conditions could fluorescence be identified in neural crest cells prior to their aggregation to form the primary sympathetic chain. Noradrenaline induced fluorescence in the neural tube, notochord, myotome, sclerotome, gut mesenchyme and suprarenal cortical cells. In addition to these structures, the dorsal pancreas and some blood cells were fluorescent after l-DOPA treatment. The implication of the results for the neural crest origin of APUD (Amine Precursor Uptake Decaboxylase) cells is considered.
利用甲醛诱导荧光法研究了16至24期鸡胚中儿茶酚胺的积累情况。荧光首先在21期出现在交感神经链前部。经左旋多巴处理后，这种荧光在18期出现。在22至24期用利血平耗尽去甲肾上腺素后，去甲肾上腺素不能提前荧光出现或恢复荧光。在神经嵴细胞聚集形成初级交感神经链之前，在任何情况下都无法在其中鉴定出荧光。去甲肾上腺素在神经管、脊索、肌节、骨节、肠间充质和肾上腺皮质细胞中诱导产生荧光。除了这些结构外，左旋多巴处理后，背侧胰腺和一些血细胞也有荧光。本文考虑了这些结果对APUD（胺前体摄取脱羧酶）细胞神经嵴起源的意义。
