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尿液中铁的测定,特别提及去铁胺试验。

Determination of iron in urine with special reference to the desferrioxamine test.

作者信息

Lundvall O, Weinfeld A

出版信息

J Clin Pathol. 1967 Jul;20(4):611-5. doi: 10.1136/jcp.20.4.611.

DOI:10.1136/jcp.20.4.611
PMID:5602575
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC473520/
Abstract

A simple and rapid method for the determination of ferrioxamine iron in urine, in which the colour reagent is added directly to centrifuged urine after reduction with dithionite, has been critically evaluated and compared with a method of iron determination after wet ashing. The coefficient of variation of both methods was less than 5%. A very high correlation was found between the values obtained with the two methods, but the simple method gave consistently lower values, averaging about 85% of those by the wet ashing method. Iron present in the urine sediment or bound in a soluble form which is not split off by dithionite will not be measured with the simple method. Despite its lower values the simple method gives uniform and reliable results, and is considered suitable for the performance of the desferrioxamine test.

摘要

一种用于测定尿中铁胺铁的简单快速方法,即在连二亚硫酸盐还原后将显色试剂直接加入离心后的尿液中,该方法已得到严格评估,并与湿灰化后铁的测定方法进行了比较。两种方法的变异系数均小于5%。发现两种方法所得值之间具有非常高的相关性,但简单方法所得值始终较低,平均约为湿灰化法所得值的85%。尿沉渣中存在的铁或以连二亚硫酸盐不能分解的可溶形式结合的铁,用简单方法无法测定。尽管其值较低,但简单方法能给出一致且可靠的结果,被认为适用于去铁胺试验。

相似文献

1
Determination of iron in urine with special reference to the desferrioxamine test.尿液中铁的测定,特别提及去铁胺试验。
J Clin Pathol. 1967 Jul;20(4):611-5. doi: 10.1136/jcp.20.4.611.
2
Simple assay for urinary iron after desferrioxamine therapy.去铁胺治疗后尿铁的简易检测方法。
Am J Clin Pathol. 1982 Mar;77(3):324-7. doi: 10.1093/ajcp/77.3.324.
3
Estimation of ferrioxamine in jaundiced urine.黄疸尿中铁胺的测定。
J Clin Pathol. 1968 Mar;21(2):169-70. doi: 10.1136/jcp.21.2.169.
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DIFFERENTIAL FERRIOXAMINE TEST FOR MEASURING CHELATABLE BODY IRON.用于测量可螯合体内铁的去铁胺差异试验。
J Clin Pathol. 1965 Jan;18(1):88-97. doi: 10.1136/jcp.18.1.88.
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Improved automated technique for the estimation of iron in ferrioxamine.用于测定去铁胺中铁含量的改进自动化技术
J Clin Pathol. 1967 May;20(3):257-9. doi: 10.1136/jcp.20.3.257.
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Rapid determination of iron in urine, in the presence of deferoxamine, by inductively coupled plasma emission spectrometry.在去铁胺存在的情况下,通过电感耦合等离子体发射光谱法快速测定尿液中的铁。
Clin Chem. 1986 Mar;32(3):521-2.
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DETERMINATION OF DESFERRIOXAMINE-B METHANE SULPHONATE IN URINE.尿中去铁胺B甲磺酸盐的测定
Clin Chim Acta. 1965 Feb;11:135-8. doi: 10.1016/0009-8981(65)90207-x.
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[Colorimetric determination of urinary iron, chelated with deferoxamine B, using a single reagent].[使用单一试剂比色法测定与去铁胺B螯合的尿铁]
Quad Sclavo Diagn. 1982 Dec;18(4):383-91.
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Modified aca method for determination of iron chelated by deferoxamine and other chelators.用于测定去铁胺和其他螯合剂螯合的铁的改良ACA方法。
Clin Chem. 1980 Oct;26(11):1593-7.
10
Determination of chelated iron in the urine.尿液中螯合铁的测定
J Clin Pathol. 1968 Mar;21(2):166-8. doi: 10.1136/jcp.21.2.166.

引用本文的文献

1
Effects of iron overload on ascorbic acid metabolism.铁过载对维生素C代谢的影响。
Br Med J. 1968 Sep 21;3(5620):704-7. doi: 10.1136/bmj.3.5620.704.

本文引用的文献

1
THE EFFECT OF DESFERRIOXAMINE ON IRON METABOLISM IN MAN.去铁胺对人体铁代谢的影响。
Scand J Haematol. 1965;2:67-79. doi: 10.1111/j.1600-0609.1965.tb01280.x.
2
EVALUATION OF DEFEROXAMINE IN IRON OVERLOAD.去铁胺在铁过载中的评估
Arch Intern Med. 1964 Dec;114:741-53. doi: 10.1001/archinte.1964.03860120053003.
3
DESFERRIOXAMINE EFFECT ON IRON EXCRETION IN HEMOCHROMATOSIS.去铁胺对血色素沉着症中铁排泄的影响。
Arch Intern Med. 1964 Mar;113:435-41. doi: 10.1001/archinte.1964.00280090121020.
4
Diagnosis of iron storage disease with desferrioxamine (Desferal test).用去铁胺(去铁胺试验)诊断铁储存疾病。
Acta Haematol. 1964 Dec;32(6):321-37. doi: 10.1159/000209579.