Pitney W R, Potter M
J Clin Pathol. 1967 Sep;20(5):710-6. doi: 10.1136/jcp.20.5.710.
Whole blood was passed through glass bead filters and the retention of platelets studied under various experimental conditions. Platelet retention was similar over a wide range of blood flow rates; however, it was decreased at fast rates of flow. Platelet retention was not influenced by the haematocrit value of the blood over a wide range. Retention in filters containing siliconized beads was similar to that in filters with untreated beads. Platelet retention was inhibited by the addition of citrate or adenosine to the blood before passage through the filter and was also less when the blood was cooled. Heparin and phenylindandione therapy did not affect the results. There was a wide range of results of platelet retention tests with normal blood samples and considerable variability between the results of duplicate samples from the same donor. Repeated testing of normal donors over a three-month period gave results which varied widely within the normal range. Twenty-two of 40 uraemic blood samples showed decreased platelet retention. The mechanism of platelet retention from whole blood by glass bead filters is not clear but may be related to platelet aggregation induced by adenosine diphosphate or other aggregating agents.
将全血通过玻璃珠过滤器，并在各种实验条件下研究血小板的滞留情况。在很宽的血流速率范围内，血小板滞留情况相似；然而，在快速血流时，血小板滞留情况会降低。在很宽的范围内，血小板滞留不受血液血细胞比容值的影响。含硅化珠的过滤器中的滞留情况与含未处理珠的过滤器中的滞留情况相似。在血液通过过滤器之前向血液中添加柠檬酸盐或腺苷可抑制血小板滞留，并且当血液冷却时血小板滞留也会减少。肝素和苯茚二酮治疗不影响结果。正常血液样本的血小板滞留测试结果范围很广，来自同一供体的重复样本结果之间存在相当大的变异性。在三个月的时间内对正常供体进行重复测试，结果在正常范围内差异很大。40份尿毒症血液样本中有22份显示血小板滞留减少。玻璃珠过滤器从全血中滞留血小板的机制尚不清楚，但可能与二磷酸腺苷或其他聚集剂诱导的血小板聚集有关。
