O'Brien J R, Shoobridge S M, Finch W J
J Clin Pathol. 1969 Jan;22(1):28-31. doi: 10.1136/jcp.22.1.28.
When whole blood was passed through a column of glass beads, heparinized and native blood gave similar results. With citrated blood far fewer platelets were lost on the column. Thus citrate inhibits this test and heparin has no effect.Platelet-rich plasma (PRP) was prepared from citrated and heparinized blood and the responses in a number of aggregation tests were compared. With added adenosine diphosphate and adrenaline (first wave), the response in heparin PRP was greater than that in citrate PRP and was proportional to the amount of free calcium ions. With added collagen and adrenaline (second wave) the response was worse in heparin than in citrate platelet-rich plasma. It is concluded that heparin has no effect on the adhesive forces but inhibits the release mechanism. This may be of therapeutic importance.
当全血通过玻璃珠柱时，肝素化血液和未处理的血液产生了相似的结果。对于枸橼酸化血液，柱上损失的血小板要少得多。因此，枸橼酸盐抑制该测试，而肝素没有影响。从枸橼酸化血液和肝素化血液中制备富血小板血浆（PRP），并比较了一些凝集试验中的反应。添加二磷酸腺苷和肾上腺素（第一波）时，肝素化PRP中的反应大于枸橼酸化PRP中的反应，且与游离钙离子的量成正比。添加胶原蛋白和肾上腺素（第二波）时，肝素化PRP中的反应比枸橼酸化富血小板血浆中的反应更差。得出的结论是，肝素对黏附力没有影响，但会抑制释放机制。这可能具有治疗意义。
