Balkwill F R, Franks C R, Oliver R T, Spector W G
J Pathol. 1977 May;122(1):13-26. doi: 10.1002/path.1711220104.
Leucocyte suspensions from some cases of acute myelogenous leukaemia produced large numbers of macrophages when cultured in vitro. The macrophages increased steadily in number during the first 1-2 wk of culture. The increase was partly derived from division of the macrophages themselves, partly from augmentation from supernatant cells not adherent to the surface of the culture flask. This augmentation occurred mostly in the first 24 hr of culture. The leukaemic macrophages had many features of normal macrophages as regards light microscopy, surface receptors, phagocytic activity and cytochemistry. Under the electron microscope, however, they had many abnormal characteristics, all of which have been recorded previously as features of malignant transformation. Cultures containing the abnormal macrophages and their precursor cells caused local solid tumours when injected into immune-deprived mice. The tumours were composed of rather uniform cells many of which resembled neoplastic reticulum cells. It is concluded that human leukaemic monocytes or promonocytes can develop into macrophages with ultrastructural features of malignancy. This appears to be the first demonstration of the development of neoplastic macrophages. It is suggested that some lymph node tumours of man may be composed of similar neoplastic macrophages and their precursor cells.
来自一些急性髓性白血病病例的白细胞悬液在体外培养时可产生大量巨噬细胞。在培养的最初1 - 2周内，巨噬细胞数量稳步增加。这种增加部分源于巨噬细胞自身的分裂，部分源于未附着于培养瓶表面的上清细胞的增殖。这种增殖大多发生在培养的最初24小时内。就光学显微镜、表面受体、吞噬活性和细胞化学而言，白血病巨噬细胞具有许多正常巨噬细胞的特征。然而，在电子显微镜下，它们有许多异常特征，所有这些特征以前都被记录为恶性转化的特征。将含有异常巨噬细胞及其前体细胞的培养物注射到免疫缺陷小鼠体内时会引起局部实体瘤。肿瘤由相当一致的细胞组成，其中许多类似于肿瘤性网状细胞。结论是人类白血病单核细胞或原单核细胞可发育为具有恶性超微结构特征的巨噬细胞。这似乎是肿瘤性巨噬细胞发育的首次证明。有人提出，人类的一些淋巴结肿瘤可能由类似的肿瘤性巨噬细胞及其前体细胞组成。
