Loewenberg J R, Chapman C M
Arch Microbiol. 1977 May 13;113(1-2):61-4. doi: 10.1007/BF00428581.
The cellulase inducer sophorose was rapidly catabolized to CO2 and H2O by Trichoderma: only small amounts were used to induce the synthesis of cellulase. 3H-sophorose uptake began after a lag of 1 h and its half-life in the medium was less than 5 h. Cellulase activity in the medium did not increase till 6 h after the addition of sophorose and reached a half maximum value at 14 h. The presence of free sophorose in the medium was required for continuous cellulase production. Several small sophorose addition induced much more cellulase than an equivalent single dose. These results are attributed to two pathways of sophorose utilization, a catabolic pathway that has a high capacity but low affinity for sophorose and an inductive pathway having a lower capacity but higher affinity for sophorose.
只有少量用于诱导纤维素酶的合成。3H-槐糖的摄取在滞后1小时后开始，其在培养基中的半衰期小于5小时。培养基中的纤维素酶活性直到添加槐糖6小时后才增加，并在14小时达到最大值的一半。培养基中游离槐糖的存在是持续产生纤维素酶所必需的。多次少量添加槐糖比等量单次添加诱导产生的纤维素酶要多得多。这些结果归因于槐糖利用的两条途径，一条分解代谢途径对槐糖具有高容量但低亲和力，一条诱导途径对槐糖具有较低容量但较高亲和力。
