丛枝异水霉游动孢子的细胞壁形成。III. 从减数孢子发育成菌丝过程中细胞壁的碳水化合物组成。

Cell wall formation in zoospores of Allomyces arbuscula. III. Carbohydrate composition of cell walls during development from meiospores to hyphae.

作者信息

Kroh M, Knuiman B, Kirby E G, Sassen M M

出版信息

Arch Microbiol. 1977 May 13;113(1-2):73-8. doi: 10.1007/BF00428583.

Abstract

Carbohydrate composition was determined in isolated cell walls of meiospores of Allomyces arbuscula after incubation for 15 min (encysted meiospores: cysts), 150 min (germlings: cysts + rhizoids) and 24 h (cysts + rhizoids + hyphae). The principal constituent in all cell wall samples is chitin, accounting for about 75% of the recovered carbohydrates. In addition, cell walls of all stages examined contain polysaccharides which release galactose, glucose, mannose, arabinose, xylose, fucose, and rhamnose on acid hydrolysis. While different developmental stages show minor quantitative changes in chitin, the ratio of galactose to glucose decreases sharply during differentiation of ungerminated cysts into germlings with rhizoids and hyphae. The increase in glucose is accompanied by a decrease in the amount of xylose and/or fucose and of galactose.

摘要

对丛枝异水霉减数分裂孢子的分离细胞壁进行培养，分别培养15分钟（包被减数分裂孢子：孢囊）、150分钟（芽管：孢囊+假根）和24小时（孢囊+假根+菌丝）后，测定其碳水化合物组成。所有细胞壁样品中的主要成分是几丁质，约占回收碳水化合物的75%。此外，所有检测阶段的细胞壁都含有多糖，这些多糖在酸水解时会释放半乳糖、葡萄糖、甘露糖、阿拉伯糖、木糖、岩藻糖和鼠李糖。虽然不同发育阶段的几丁质含量有微小的定量变化，但在未萌发的孢囊分化为带有假根和菌丝的芽管过程中，半乳糖与葡萄糖的比例急剧下降。葡萄糖含量的增加伴随着木糖和/或岩藻糖以及半乳糖含量的减少。