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The charge-relay system of serine proteinases: proton magnetic resonance titration studies of the four histidines of porcine trypsin.

作者信息

Markley J L, Porubcan M A

出版信息

J Mol Biol. 1976 Apr 15;102(3):487-509. doi: 10.1016/0022-2836(76)90330-2.

DOI:10.1016/0022-2836(76)90330-2
PMID:5610
Abstract
摘要

相似文献

1
The charge-relay system of serine proteinases: proton magnetic resonance titration studies of the four histidines of porcine trypsin.
J Mol Biol. 1976 Apr 15;102(3):487-509. doi: 10.1016/0022-2836(76)90330-2.
2
(Diisopropylphosphoryl)serine proteinases. Proton and phosphorus-31 nuclear magnetic resonance-pH titration studies.
Biochemistry. 1979 Sep 18;18(19):4108-16. doi: 10.1021/bi00586a008.
3
Zymogen activation in serine proteinases. Proton magnetic resonance pH titration studies of the two histidines of bovine chymotrypsinogen A and chymotrypsin Aalpha.丝氨酸蛋白酶中的酶原激活。牛胰凝乳蛋白酶原A和α-胰凝乳蛋白酶的两个组氨酸的质子磁共振pH滴定研究。
Biochemistry. 1978 Oct 31;17(22):4627-40. doi: 10.1021/bi00615a008.
4
The active centers of serine proteinases.丝氨酸蛋白酶的活性中心。
Ann N Y Acad Sci. 1974 Feb 18;227:438-45. doi: 10.1111/j.1749-6632.1974.tb14406.x.
5
Carbon nuclear magnetic resonance studies of the histidine residue in alpha-lytic protease. Implications for the catalytic mechanism of serine proteases.α-裂解蛋白酶中组氨酸残基的碳核磁共振研究。对丝氨酸蛋白酶催化机制的启示。
Biochemistry. 1973 Nov 6;12(23):4732-43. doi: 10.1021/bi00747a028.
6
Is the serine protease "charge relay system" a charge relay system?丝氨酸蛋白酶的“电荷中继系统”是一种电荷中继系统吗？
Biochem Biophys Res Commun. 1975 Mar 3;63(1):78-83. doi: 10.1016/s0006-291x(75)80013-1.
7
Hydrogen bonds in serine proteinases and their complexes with protein proteinase inhibitors. Proton nuclear magnetic resonance studies.
Biochemistry. 1978 Oct 31;17(22):4648-56. doi: 10.1021/bi00615a010.
8
Active site in zymogens. Proton magnetic resonance pH titration curves of histidine-57 in porcine and bovine trypsinogens and in their complexes with bovine pancreatic trypsin inhibitor (Kunitz).酶原中的活性位点。猪和牛胰蛋白酶原及其与牛胰蛋白酶抑制剂（库尼茨）复合物中组氨酸-57的质子磁共振pH滴定曲线。
Biochemistry. 1978 Oct 31;17(22):4640-7. doi: 10.1021/bi00615a009.
9
High resolution NMR study of the charge relay system in chymotrypsin.胰凝乳蛋白酶中电荷中继系统的高分辨率核磁共振研究。
Ann N Y Acad Sci. 1973 Dec 31;222:220-5. doi: 10.1111/j.1749-6632.1973.tb15263.x.
10
Proton magnetic resonance titration curves of the three histidine residues of staphylococcal protease.葡萄球菌蛋白酶三个组氨酸残基的质子磁共振滴定曲线
Biochemistry. 1975 Mar 11;14(5):998-1005. doi: 10.1021/bi00676a019.

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Tethered imidazole mediated duplex stabilization and its potential for aptamer stabilization.束缚咪唑介导的双链体稳定及其对适体稳定的潜在作用。
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Biomolecular NMR: Past and future.生物分子核磁共振：过去与未来。
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Identification of a pKa-regulating motif stabilizing imidazole-modified double-stranded DNA.一种稳定咪唑修饰双链DNA的pKa调节基序的鉴定。
Nucleic Acids Res. 2015 Jan;43(1):51-62. doi: 10.1093/nar/gku1306. Epub 2014 Dec 17.
4
The catalytic aspartate is protonated in the Michaelis complex formed between trypsin and an in vitro evolved substrate-like inhibitor: a refined mechanism of serine protease action.在胰蛋白酶与体外进化的底物类似抑制剂形成的米氏复合物中，催化天冬氨酸被质子化：丝氨酸蛋白酶作用的精细机制。
J Biol Chem. 2011 Feb 4;286(5):3587-96. doi: 10.1074/jbc.M110.161604. Epub 2010 Nov 21.
5
Unusual 1H NMR chemical shifts support (His) C(epsilon) 1...O==C H-bond: proposal for reaction-driven ring flip mechanism in serine protease catalysis.异常的¹H NMR化学位移支持（组氨酸）C(ε)1...O==C氢键：丝氨酸蛋白酶催化中反应驱动环翻转机制的提议。
Proc Natl Acad Sci U S A. 2000 Sep 12;97(19):10371-6. doi: 10.1073/pnas.97.19.10371.
6
Catalytic mechanism of serine proteases: reexamination of the pH dependence of the histidyl 1J13C2-H coupling constant in the catalytic triad of alpha-lytic protease.丝氨酸蛋白酶的催化机制：对α-裂解蛋白酶催化三联体中组氨酸1J13C2-H耦合常数pH依赖性的重新审视。
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7323-6. doi: 10.1073/pnas.78.12.7323.
7
Charge state of His-57-Asp-102 couple in a transition state analogue-trypsin complex: a molecular orbital study.过渡态类似物-胰蛋白酶复合物中His-57-Asp-102对的电荷状态：分子轨道研究
Proc Natl Acad Sci U S A. 1984 Oct;81(20):6266-70. doi: 10.1073/pnas.81.20.6266.
8
Confirmation of the assignment of the low-field proton resonance of serine proteases by using specifically nitrogen-15 labeled enzyme.通过使用特定的氮-15标记酶来确认丝氨酸蛋白酶低场质子共振的归属。
Proc Natl Acad Sci U S A. 1985 Dec;82(23):7948-51. doi: 10.1073/pnas.82.23.7948.
9
Application of pulse radiolysis to the study of proteins: chymotrypsin and trypsin.脉冲辐解技术在蛋白质研究中的应用：胰凝乳蛋白酶和胰蛋白酶。
Biophys J. 1978 Oct;24(1):307-17. doi: 10.1016/S0006-3495(78)85379-X.
10
Inhibition of alpha-chymotrypsin by 2-halogeno-ethanols; comparisons with 2-methyl-ethanols and urea.2-卤代乙醇对α-糜蛋白酶的抑制作用；与2-甲基乙醇和尿素的比较。
Experientia. 1979 Aug 15;35(8):1011-2. doi: 10.1007/BF01949911.