Webb C G, Gall W E, Edelman G M
J Exp Med. 1977 Oct 1;146(4):923-32. doi: 10.1084/jem.146.4.923.
Synthesis of H-2 antigens by preimplantation mouse embryos is first detectable at the late blastocyst stage. These antigens were detected using immune precipitation assays of extracts of embryos labeled by incorporation of radioactive amino acids but not by surface iodination. Experiments using isolated inner cell massess and trophoblast vesicles indicate that it is the cells of the inner cell mass that synthesize these antigens. H-2 antigens were not detected in either early blastocysts or at earlier cleavage stages.
植入前小鼠胚胎合成H-2抗原最早在囊胚晚期可检测到。这些抗原是通过对掺入放射性氨基酸标记的胚胎提取物进行免疫沉淀测定来检测的，而不是通过表面碘化来检测。使用分离的内细胞团和滋养层小泡进行的实验表明，是内细胞团的细胞合成了这些抗原。在早期囊胚或更早的卵裂阶段均未检测到H-2抗原。
