Flexner J B, Flexner L B, Hoffman P L, Walter R
Brain Res. 1977 Sep 23;134(1):139-44. doi: 10.1016/0006-8993(77)90931-3.
Intracerebral injections of puromycin one day after training of mice in a Y-maze cause amnesia when the animals are tested 7 days later. This amnesia was shown to be attenuated by various neurohypophyseal hormones, analogs and fragments, administered subcutaneously immediately after training. Dose-response relationships have been obtained for the attenuation of puromycin-induced amnesia in mice by selected neurohypophyseal peptides. All of the compounds tested reduce the amnesia in a dose-related way, suggesting that these peptides may interact with specific receptors to induce their central effect. Among the peptides studied the two most potent--i.e., those that cause substantial retention of memory at the lowest doses--are the neurohypophyseal hormone arginine vasopressin and Z-prolyl-leucyl-glycinamide (Z-MIF).
在小鼠于Y型迷宫中训练一天后，向其脑内注射嘌呤霉素，当7天后对动物进行测试时会导致失忆。研究表明，在训练后立即皮下注射各种神经垂体激素、类似物和片段可减轻这种失忆。已获得特定神经垂体肽减轻嘌呤霉素诱导的小鼠失忆的剂量反应关系。所有测试的化合物均以剂量相关的方式减少失忆，这表明这些肽可能与特定受体相互作用以诱导其中枢效应。在所研究的肽中，两种最有效的——即在最低剂量下能使记忆大量保留的——是神经垂体激素精氨酸加压素和Z-脯氨酰-亮氨酰-甘氨酰胺（Z-MIF）。
