Kelly D P, Dewar M K, Johns R B, Wei-Let S, Yates J F
Adv Exp Med Biol. 1977;86A:641-7. doi: 10.1007/978-1-4684-3282-4_38.
Model cross-linked systems have been prepared by reacting amino acids or alkylamines with formaldehyde and various amino acid model compounds such as 2,4-dimethylphenol (tyrosine), 3-methylindole (tryptophan) and alkylamides (glutamine, asparagine. 13C NMR spectra of the products show the resonances of the formaldehyde-derived methylene carbons in the region 45-60 ppm. Interferences occur from resonances of the alpha-amino acid methine carbons. From the data for these products and other model compounds it has been possible to predict the shifts of the residual methylene carbons in a variety of cross-linked systems. This NMR technique shows promise as a rapid non-degradative method for identification of cross-linking sites.
通过使氨基酸或烷基胺与甲醛以及各种氨基酸模型化合物（如2,4-二甲基苯酚（酪氨酸）、3-甲基吲哚（色氨酸）和烷基酰胺（谷氨酰胺、天冬酰胺））反应，制备了模型交联体系。产物的13C NMR光谱显示了在45 - 60 ppm区域内甲醛衍生的亚甲基碳的共振信号。α-氨基酸次甲基碳的共振信号会产生干扰。根据这些产物和其他模型化合物的数据，已能够预测各种交联体系中残余亚甲基碳的化学位移。这种NMR技术有望成为一种用于识别交联位点的快速非降解方法。
