Stenstad P, Ostgaard K, Eik-nes K B
Mol Cell Endocrinol. 1977 Oct;8(4):283-9. doi: 10.1016/0303-7207(77)90003-x.
The metabolism of radioactive testosterone, 5alpha-dihydrotestosterone, 4-androstene-3beta,17beta-diol or 4-androstene-3alpha,17beta-diol by the human cell line NHIK 3025, derived from a carcinoma of the uterine cervix, was studied. The cells were grown in Eagle's minimal essential medium (MEM) with a steriod concentration of 10-(7) M for 4 days. Androgen metabolism by this cell line is essentially the same as for other androgen-responsive cells. The most interesting testosterone metabolite in this system is 4-androstene-3beta,17beta-diol, and the separation of this compound from 4-androstene-3alpha,17beta-diol and the two corresponding 5alpha-reduced diols is described. Since 4-androsterone-3beta,17beta-diol is a more potent growth factor for these cells than testosterone, the small conversion of testosterone to 4-androstene-3beta, 17beta-diol observed could be responsible for the growth stimulation by testosterone.
对源自子宫颈癌的人类细胞系NHIK 3025中放射性睾酮、5α-二氢睾酮、4-雄烯-3β,17β-二醇或4-雄烯-3α,17β-二醇的代谢进行了研究。细胞在含有10-(7)M类固醇浓度的伊格尔氏基本培养基（MEM）中培养4天。该细胞系的雄激素代谢与其他雄激素反应性细胞基本相同。该系统中最有趣的睾酮代谢产物是4-雄烯-3β,17β-二醇，并描述了该化合物与4-雄烯-3α,17β-二醇以及两种相应的5α-还原二醇的分离方法。由于4-雄甾酮-3β,17β-二醇对这些细胞而言是比睾酮更有效的生长因子，所观察到的睾酮向4-雄烯-3β,17β-二醇的少量转化可能是睾酮刺激生长的原因。
