Wibe E, Ostgaard K, Eik-Nes K B
Mol Cell Endocrinol. 1976 Oct;5(5):359-64. doi: 10.1016/0303-7207(76)90018-6.
The effect of different steroids on the growth of a human cell line, was examined in NHIK 3025 cells, derived from a carcinoma of the uterine cervix. When this cell line was grown in Eagle's minimal essential medium (MEM) for 4 days, addition to the medium of estradiol-17 beta in concentrations ranging from 10(-9) to 10(-7) M did not promote significant cell growth. Stimulated growth was observed with testosterone in these concentrations, with maximal effect (29% above control) at 10(-7) M. 5alpha-Dihydrotestosterone over the range 10(-9)--10(-6) M gave no such effect. 4-Androstene-3,17-dione, 5alpha-androstan-3alpha,17beta-diol and 5alpha-androstan-3beta,17beta-diol had no significant effect on cell proliferation at a concentration of 10(-7) M, while 4-androstene-3beta,17beta-diol augmented cell number to values 50-60% higher than those of control cultures. The observed increase in cell number was due to a shortening of the cell cycle and to a higher plating efficiency. Thus, cell line NHIK 3025 has not the responsiveness to estradiol-17beta of normal uterine cells. The data suggest that testosterone and/or 4-androstene-3beta,17beta-diol may be growth factors for this cell line.
在源自子宫颈癌的NHIK 3025细胞中，研究了不同类固醇对人细胞系生长的影响。当该细胞系在伊格尔氏基本培养基（MEM）中培养4天时，向培养基中添加浓度范围为10^(-9)至10^(-7)M的17β-雌二醇并不能促进细胞显著生长。在这些浓度下，观察到睾酮能刺激生长，在10^(-7)M时效果最佳（比对照高29%）。10^(-9) - 10^(-6)M范围内的5α-双氢睾酮没有这种作用。在浓度为10^(-7)M时，4-雄烯-3,17-二酮、5α-雄甾烷-3α,17β-二醇和5α-雄甾烷-3β,17β-二醇对细胞增殖没有显著影响，而4-雄烯-3β,17β-二醇使细胞数量增加到比对照培养物高50 - 60%的值。观察到的细胞数量增加是由于细胞周期缩短和接种效率提高。因此，细胞系NHIK 3025对正常子宫细胞的17β-雌二醇没有反应性。数据表明，睾酮和/或4-雄烯-3β,17β-二醇可能是该细胞系的生长因子。
