平滑肌肌球蛋白组装成侧极丝。
Assembly of smooth muscle myosin into side-polar filaments.
作者信息
Craig R, Megerman J
出版信息
J Cell Biol. 1977 Dec;75(3):990-6. doi: 10.1083/jcb.75.3.990.
Abstract
The in vitro assembly of myosin purified from calf aorta muscle has been studied by electron microscopy. Two types of filament are formed: short bipolar filament similar to those formed from skeletal muscle myosin, and longer "side-polar" filaments having cross bridges with a single polarity along the entire length of one side and the opposite polarity along the other side. Unlike the case with skeletal myosin filaments, antiparallel interactions between myosin molecules occur along the whole length of side-polar filaments. The side-polar structure may be related to the in vivo form of myosin in vertebrate smooth muscle.
摘要
通过电子显微镜对从小牛主动脉肌肉中纯化的肌球蛋白进行了体外组装研究。形成了两种类型的细丝：类似于由骨骼肌肌球蛋白形成的短双极细丝，以及较长的“侧极”细丝，其沿一侧的整个长度具有单一极性的横桥，而沿另一侧具有相反的极性。与骨骼肌肌球蛋白细丝的情况不同，肌球蛋白分子之间的反平行相互作用沿着侧极细丝的整个长度发生。侧极结构可能与脊椎动物平滑肌中肌球蛋白的体内形式有关。
相似文献
1
Assembly of smooth muscle myosin into side-polar filaments.平滑肌肌球蛋白组装成侧极丝。
J Cell Biol. 1977 Dec;75(3):990-6. doi: 10.1083/jcb.75.3.990.
2
Myosin filament structure in vertebrate smooth muscle.脊椎动物平滑肌中的肌球蛋白丝结构。
J Cell Biol. 1996 Jul;134(1):53-66. doi: 10.1083/jcb.134.1.53.
3
Myosin filaments isolated from skinned amphibian smooth muscle cells are side-polar.从去皮两栖类平滑肌细胞中分离出的肌球蛋白丝是侧极的。
J Muscle Res Cell Motil. 1989 Jun;10(3):206-20. doi: 10.1007/BF01739811.
4
Assembly of smooth muscle myosin minifilaments: effects of phosphorylation and nucleotide binding.平滑肌肌球蛋白微丝的组装：磷酸化和核苷酸结合的影响。
J Cell Biol. 1987 Dec;105(6 Pt 2):3007-19. doi: 10.1083/jcb.105.6.3007.
5
Polylysine binding to unphosphorylated smooth muscle myosin enhances formation and stabilizes myosin filaments in vitro.
Acta Physiol Scand. 2002 Apr;174(4):337-46. doi: 10.1046/j.1365-201x.2002.00950.x.
6
Mass determination of native smooth muscle myosin filaments by scanning transmission electron microscopy.通过扫描透射电子显微镜对天然平滑肌肌球蛋白丝进行质量测定。
J Mol Biol. 2002 May 10;318(4):999-1007. doi: 10.1016/S0022-2836(02)00191-2.
7
Smitin, a novel smooth muscle titin-like protein, interacts with myosin filaments in vivo and in vitro.斯米汀是一种新型的平滑肌肌联蛋白样蛋白，在体内和体外均能与肌球蛋白丝相互作用。
J Cell Biol. 2002 Jan 7;156(1):101-11. doi: 10.1083/jcb.200107037.
8
Electron microscopy of synthetic myosin filaments. Evidence for cross-bridge. Flexibility and copolymer formation.合成肌球蛋白丝的电子显微镜观察。横桥的证据。柔韧性和共聚物形成。
J Cell Biol. 1975 Oct;67(1):93-104. doi: 10.1083/jcb.67.1.93.
9
Studies on isolated smooth muscle cells: The contractile apparatus.对分离平滑肌细胞的研究：收缩装置。
J Cell Sci. 1977 Apr;24:327-49. doi: 10.1242/jcs.24.1.327.
10
Muscle myosins form folded monomers, dimers, and tetramers during filament polymerization in vitro.在体外肌球蛋白丝聚合过程中，肌球蛋白折叠形成单体、二聚体和四聚体。
Proc Natl Acad Sci U S A. 2020 Jul 7;117(27):15666-15672. doi: 10.1073/pnas.2001892117. Epub 2020 Jun 22.
引用本文的文献
1
Impacts of structural properties of myosin II filaments on force generation.肌球蛋白II细丝的结构特性对力产生的影响。
Elife. 2025 Aug 13;14:RP105236. doi: 10.7554/eLife.105236.
2
John Squire and the myosin thick filament structure in muscle.约翰·斯奎尔与肌肉中的肌球蛋白粗丝结构。
J Muscle Res Cell Motil. 2023 Sep;44(3):143-152. doi: 10.1007/s10974-023-09646-4. Epub 2023 Apr 26.
3
Myosin assembly of smooth muscle: from ribbons and side polarity to a row polar helical model.平滑肌肌球蛋白组装：从带状和侧向极性到排极螺旋模型。
J Muscle Res Cell Motil. 2022 Sep;43(3):113-133. doi: 10.1007/s10974-022-09622-4. Epub 2022 Jul 16.
4
Self-assembly of smooth muscle myosin filaments: adaptation of filament length by telokin and Mg·ATP.平滑肌肌球蛋白丝的自组装：telokin 和 Mg·ATP 通过适应丝长。
Eur Biophys J. 2022 Sep;51(6):449-463. doi: 10.1007/s00249-022-01608-x. Epub 2022 Jul 12.
5
The myosin II coiled-coil domain atomic structure in its native environment.肌球蛋白 II 卷曲螺旋结构域的原子结构在其天然环境中。
Proc Natl Acad Sci U S A. 2021 Apr 6;118(14). doi: 10.1073/pnas.2024151118.
6
Filament evanescence of myosin II and smooth muscle function.肌球蛋白 II 的丝消失和平滑肌功能。
J Gen Physiol. 2021 Mar 1;153(3). doi: 10.1085/jgp.202012781.
7
Evidence for S2 flexibility by direct visualization of quantum dot-labeled myosin heads and rods within smooth muscle myosin filaments moving on actin in vitro.通过直接可视化体外肌球蛋白丝上肌球蛋白头部和杆部的量子点标记，以及在肌动蛋白上运动的肌球蛋白，证明 S2 的柔韧性。
J Gen Physiol. 2021 Mar 1;153(3). doi: 10.1085/jgp.202012751.
8
Muscle myosins form folded monomers, dimers, and tetramers during filament polymerization in vitro.在体外肌球蛋白丝聚合过程中，肌球蛋白折叠形成单体、二聚体和四聚体。
Proc Natl Acad Sci U S A. 2020 Jul 7;117(27):15666-15672. doi: 10.1073/pnas.2001892117. Epub 2020 Jun 22.
9
Myosin Crossbridge, Contractile Unit, and the Mechanism of Contraction in Airway Smooth Muscle: A Mechanical Engineer's Perspective.肌球蛋白横桥、收缩单位与气道平滑肌收缩机制：机械工程师视角
J Eng Sci Med Diagn Ther. 2019 Feb;2(1):0108041-108046. doi: 10.1115/1.4042479. Epub 2019 Feb 8.
10
Ordering of myosin II filaments driven by mechanical forces: experiments and theory.机械力驱动的肌球蛋白 II 丝的排序：实验与理论。
Philos Trans R Soc Lond B Biol Sci. 2018 May 26;373(1747). doi: 10.1098/rstb.2017.0114.
本文引用的文献
1
Aggregation of thick filaments into ribbons in mammalian smooth muscle.哺乳动物平滑肌中粗肌丝聚集成带。
Nat New Biol. 1971 Jun 23;231(25):243-6. doi: 10.1038/newbio231243a0.
2
Filaments and ribbons in vertebrate smooth muscle.脊椎动物平滑肌中的细丝和带状物。
Nature. 1974 May 10;249(453):152-4. doi: 10.1038/249152a0.
3
Purification of uterine myosin and synthetic filament formation.子宫肌球蛋白的纯化及合成丝的形成
J Mol Biol. 1974 Sep 15;88(2):385-91. doi: 10.1016/0022-2836(74)90489-6.
4
Filament formation by slime mould myosin isolated at low ionic strength.
J Cell Sci. 1974 Jun;15(1):113-29. doi: 10.1242/jcs.15.1.113.
5
Isolation and characterization of myosin from cloned mouse fibroblasts.从小鼠克隆成纤维细胞中分离和鉴定肌球蛋白。
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3693-7. doi: 10.1073/pnas.69.12.3693.
6
Potassium accumulation in smooth muscle and associated ultrastructural changes.钾在平滑肌中的蓄积及相关超微结构变化。
J Physiol. 1973 Jul;232(2):247-73. doi: 10.1113/jphysiol.1973.sp010268.
7
Filament organization in vertebrate smooth muscle.
Philos Trans R Soc Lond B Biol Sci. 1973 Mar 15;265(867):223-9. doi: 10.1098/rstb.1973.0027.
8
The assembly of ribbon-shaped structures in low ionic strength extracts obtained from vertebrate smooth muscle.从脊椎动物平滑肌获得的低离子强度提取物中带状结构的组装。
Philos Trans R Soc Lond B Biol Sci. 1973 Mar 15;265(867):203-12. doi: 10.1098/rstb.1973.0024.
9
Structural basis of contraction in vertebrate smooth muscle.脊椎动物平滑肌收缩的结构基础。
J Mol Biol. 1972 Jun 14;67(1):117-49. doi: 10.1016/0022-2836(72)90390-7.
10
Segments from vertebrate smooth muscle myosin rods.
J Mol Biol. 1971 Aug 14;59(3):527-9. doi: 10.1016/0022-2836(71)90316-0.
Zotero 插件