干扰大鼠组并将它们单独关笼饲养后脂肪酸和色氨酸的变化。

Fatty acid and tryptophan changes on disturbing groups of rats and caging them singly.

作者信息

Knott P J, Hutson P H, Curzon G

出版信息

Pharmacol Biochem Behav. 1977 Sep;7(3):245-52. doi: 10.1016/0091-3057(77)90141-1.

Abstract

The effects of disturbing groups of 24 hr fasted rats on plasma unesterified fatty acid (UFA) and tryptophan concentrations and brain tryptophan concentrations were investigated. Removing rats from cages rapidly increased plasma UFA and corticosterone and decreased plasma and whole blood tryptophan of cage mates. The disturbance also appeared to influence biochemical values of rats in other cages within the same chamber. Effects specific to individual cages were also suggested. In subsequent experiments 24 fasting rats caged together were rapidly transferred to 24 separate cages and killed at intervals. Plasma UFA rose to a maximum by 12 min and then fell toward initial values. Plasma total tryptophan concurrently fell then rose. Its percentage in the free (ultrafilterable) state, and in some experiments the absolute values of free tryptophan rose then fell. When the latter rise was marked then brain tryptophan and the 5-HT metabolite 5-hydroxyindoleacetic acid rose. Tyrosine changes were negligible. Thus altered brain tryptophan level and 5-HT metabolism may be associated with plasma tryptophan changes caused by brief environmental disturbance.

摘要

研究了干扰禁食24小时大鼠组对血浆非酯化脂肪酸（UFA）、色氨酸浓度及脑色氨酸浓度的影响。将大鼠迅速从笼中移出会使血浆UFA和皮质酮增加，同时降低笼中其他大鼠的血浆和全血色氨酸水平。这种干扰似乎也会影响同一实验室内其他笼中大鼠的生化指标。实验还提示了各个笼子所特有的效应。在后续实验中，将24只一起饲养的禁食大鼠迅速转移到24个单独的笼子中，并每隔一段时间处死一批。血浆UFA在12分钟时升至最高，随后降至初始值。血浆总色氨酸先下降后上升。其游离（可超滤）状态下的百分比，在某些实验中游离色氨酸的绝对值先上升后下降。当后者的上升明显时，脑色氨酸和5-羟色胺代谢物5-羟吲哚乙酸会上升。酪氨酸变化可忽略不计。因此，短暂环境干扰引起的血浆色氨酸变化可能与脑色氨酸水平及5-羟色胺代谢的改变有关。