Malinow M R, McLaughlin P, Papworth L, Stafford C, Kohler G O, Livingston A L, Cheeke P R
Am J Clin Nutr. 1977 Dec;30(12):2061-7. doi: 10.1093/ajcn/30.12.2061.
Five to 20 mg of saponins obtained from alfalfa tops or roots were introduced intragastrically in rats also receiving oral and intravenous ring-labeled cholesterol. The saponins were tested before and after partial acid hydrolysis. Absorption of cholesterol was determined by estimation of fecal sterols and by a dual isotope technique involving assay of plasma radioactivity. Alfalfa top saponins (nonhydrolyzed) reduced absorption of cholesterol. Acid hydrolysis of alfalfa top or root saponins enhanced their ability to inhibit cholesterol absorption.
将从紫花苜蓿顶部或根部提取的5至20毫克皂苷经胃内途径给予同样接受口服和静脉注射环状标记胆固醇的大鼠。在部分酸水解前后对皂苷进行了测试。通过粪便固醇的测定以及涉及血浆放射性测定的双同位素技术来确定胆固醇的吸收情况。紫花苜蓿顶部皂苷（未水解）降低了胆固醇的吸收。紫花苜蓿顶部或根部皂苷的酸水解增强了它们抑制胆固醇吸收的能力。
