分化肌细胞培养物中原肌球蛋白的合成。
Sythesis of tropomyosin in cultures of differentiating muscle cells.
作者信息
Allen R E, Stromer M H, Goll D E, Robson R M
出版信息
J Cell Biol. 1978 Jan;76(1):98-104. doi: 10.1083/jcb.76.1.98.
Abstract
The accumulation of tropomyosin in cultures of differentiating muscle cells was quantitatively measured. Tropomyosin was isolated from cultured cells during and after myoblast fusion; both alpha- and beta-subunits were present in myotube cultures. During fusion small amounts of tropomyosin were detectable, but, as fusion approached a maximum, tropomyosin accumulation began to increase. The increased synthesis of tropomyosin after the initiation of muscle cell fusion is consistent with the increased synthesis of other proteins characteristic of muscle, including myosin.
摘要
对分化中的肌细胞培养物中原肌球蛋白的积累进行了定量测定。在成肌细胞融合期间及之后，从培养细胞中分离出原肌球蛋白；肌管培养物中同时存在α-亚基和β-亚基。在融合过程中可检测到少量原肌球蛋白，但随着融合接近最大值，原肌球蛋白的积累开始增加。肌肉细胞融合开始后原肌球蛋白合成的增加与包括肌球蛋白在内的其他肌肉特征性蛋白质合成的增加是一致的。
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