Tepperman K, Essien F, Heywood S M
J Cell Physiol. 1975 Dec;86(3 Pt 1):553-60. doi: 10.1002/jcp.1040860312.
The results reported here have shown that there are significant differences between polysome patterns obtained from cultured cells and from freshly isolated muscle tissue. Polysomes from embryonic homogenates show different patterns with different levels of myosin synthesis, but this does not appear to be the case with cultured cells. Experiments utilizing cell-free protein synthesizing systems indicate that the polysomes isolated from myoblast cultures can synthesize myosin at levels similar to those obtained from myotube cultures, suggesting that the myoblasts contain significant amounts of the messenger RNA for myosin. In contrast, the polysomes isolated from BrdUrd-inhibited cultures synthesize a comparatively low level of myosin. These findings illustrate a significant difference between myoblasts and BrdUrd-inhibited cells.
此处报告的结果表明，从培养细胞和新鲜分离的肌肉组织中获得的多核糖体模式存在显著差异。胚胎匀浆中的多核糖体显示出不同的模式以及不同水平的肌球蛋白合成，但培养细胞似乎并非如此。利用无细胞蛋白质合成系统进行的实验表明，从成肌细胞培养物中分离出的多核糖体能够以与从肌管培养物中获得的水平相似的水平合成肌球蛋白，这表明成肌细胞含有大量的肌球蛋白信使核糖核酸。相比之下，从溴脱氧尿苷抑制的培养物中分离出的多核糖体合成的肌球蛋白水平相对较低。这些发现说明了成肌细胞与溴脱氧尿苷抑制细胞之间的显著差异。
