用于植物细胞悬浮液连续培养和自动采样的植物培养装置

Phytostat for continuous culture and automatic sampling of plant-cell suspensions.

作者信息

Miller R A, Shyluk J P, Gamborg O L, Kirkpatrick J W

出版信息

Science. 1968 Feb 2;159(3814):540-2. doi: 10.1126/science.159.3814.540.

DOI:10.1126/science.159.3814.540

Abstract

An apparatus for growing plant cells in suspension culture is described; it may be used for continuous or batch culture, and is equipped with a valve for automatic collection of samples. Aeration is by continuous bubbling of air into the culture through fritted glass. Normal culture-duplication times are from 30 to 35 hours.

摘要

描述了一种用于悬浮培养植物细胞的装置；它可用于连续培养或分批培养，并配备有用于自动采集样品的阀门。通过烧结玻璃向培养物中连续鼓入空气进行通气。正常的培养倍增时间为30至35小时。