Stokes J L, Larkin J M
J Bacteriol. 1968 Jan;95(1):95-8. doi: 10.1128/jb.95.1.95-98.1968.
We investigated the influence of temperature, in the range of 45 to 5 C, on the rate of oxidation of glucose and citrate by intact cells and cell-free extracts of psychrophilic Bacillus psychrophilus and mesophilic B. thuringiensis. Both glucose and endogenous oxidation by whole and disrupted cells of the psychrophile decreased more slowly with decrease in temperature than did glucose and endogenous oxidation by whole and disrupted cells of the mesophile. Similar results were obtained for the oxidation of citrate by cell-free extracts. Since substrate permeability is not involved in the oxidative metabolism of the cell-free extracts, we concluded that the internal enzymes of the psychrophile differ from those of the mesophile.
我们研究了在45至5摄氏度范围内温度对嗜冷性嗜冷芽孢杆菌和嗜温性苏云金芽孢杆菌的完整细胞及无细胞提取物氧化葡萄糖和柠檬酸盐速率的影响。嗜冷菌完整细胞和破碎细胞对葡萄糖的氧化以及内源性氧化,随温度降低而下降的速度比嗜温菌完整细胞和破碎细胞对葡萄糖的氧化以及内源性氧化的下降速度更慢。无细胞提取物氧化柠檬酸盐也得到了类似结果。由于底物通透性不参与无细胞提取物的氧化代谢,我们得出结论,嗜冷菌的内部酶与嗜温菌的不同。