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温度对嗜冷芽孢杆菌和嗜温芽孢杆菌全细胞及破碎细胞氧化代谢的比较影响

Comparative effect of temperature on the oxidative metabolism of whole and disrupted cells of a psychrophilic and a mesophilic species of Bacillus.

作者信息

Stokes J L, Larkin J M

出版信息

J Bacteriol. 1968 Jan;95(1):95-8. doi: 10.1128/jb.95.1.95-98.1968.

Abstract

We investigated the influence of temperature, in the range of 45 to 5 C, on the rate of oxidation of glucose and citrate by intact cells and cell-free extracts of psychrophilic Bacillus psychrophilus and mesophilic B. thuringiensis. Both glucose and endogenous oxidation by whole and disrupted cells of the psychrophile decreased more slowly with decrease in temperature than did glucose and endogenous oxidation by whole and disrupted cells of the mesophile. Similar results were obtained for the oxidation of citrate by cell-free extracts. Since substrate permeability is not involved in the oxidative metabolism of the cell-free extracts, we concluded that the internal enzymes of the psychrophile differ from those of the mesophile.

摘要

我们研究了在45至5摄氏度范围内温度对嗜冷性嗜冷芽孢杆菌和嗜温性苏云金芽孢杆菌的完整细胞及无细胞提取物氧化葡萄糖和柠檬酸盐速率的影响。嗜冷菌完整细胞和破碎细胞对葡萄糖的氧化以及内源性氧化,随温度降低而下降的速度比嗜温菌完整细胞和破碎细胞对葡萄糖的氧化以及内源性氧化的下降速度更慢。无细胞提取物氧化柠檬酸盐也得到了类似结果。由于底物通透性不参与无细胞提取物的氧化代谢,我们得出结论,嗜冷菌的内部酶与嗜温菌的不同。

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本文引用的文献

3
SUGAR TRANSPORT IN A PSYCHROPHILIC YEAST.嗜冷酵母中的糖转运
J Bacteriol. 1963 Dec;86(6):1259-64. doi: 10.1128/jb.86.6.1259-1264.1963.
7
Taxonomy of psychrophilic strains of Bacillus.嗜冷芽孢杆菌菌株的分类学
J Bacteriol. 1967 Oct;94(4):889-95. doi: 10.1128/jb.94.4.889-895.1967.
8
Heat-labile enzymes in a psychrophilic bacterium.嗜冷细菌中的热不稳定酶。
J Bacteriol. 1967 Jan;93(1):199-206. doi: 10.1128/jb.93.1.199-206.1967.
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Isolation of psychrophilic species of Bacillus.嗜冷芽孢杆菌物种的分离
J Bacteriol. 1966 May;91(5):1667-71. doi: 10.1128/jb.91.5.1667-1671.1966.

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