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从牛瘤胃中分离出的产粪臭素乳酸杆菌的特性研究

Isolation and characteristics of a skatole-producing Lactobacillus sp. from the bovine rumen.

作者信息

Yokoyama M T, Carlson J R, Holdeman L V

出版信息

Appl Environ Microbiol. 1977 Dec;34(6):837-42. doi: 10.1128/aem.34.6.837-842.1977.

DOI:10.1128/aem.34.6.837-842.1977
PMID:563703
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC242757/
Abstract

A bacterium that is capable of decarboxylating indoleacetic acid to skatole (3-methylindole) has been isolated from an L-tryptophan enrichment of bovine rumen fluid. The bacterium is a gram-positive, nonmotile, nonsporeforming rod. It is an obligate anaerobe, and strains predominatly produce D-(-)-lactic acid, with smaller amounts of L-(+)-lactic acid and acetic acid, from sugar. All four strains isolated gave a negative reaction to the indole test because they cannot form skatole directly from tryptophan. This is the first report of indoleacetic acid decarboxylation to skatole in pure culture and the demonstration of skatole production by a Lactobacillus species.

摘要

从富含L-色氨酸的牛瘤胃液中分离出一种能够将吲哚乙酸脱羧生成粪臭素(3-甲基吲哚)的细菌。该细菌为革兰氏阳性、不运动、不产芽孢的杆菌。它是专性厌氧菌,菌株主要从糖类产生D-(-)-乳酸,同时产生少量的L-(+)-乳酸和乙酸。分离出的所有四株菌株对吲哚试验均呈阴性反应,因为它们不能直接从色氨酸形成粪臭素。这是关于吲哚乙酸在纯培养中脱羧生成粪臭素以及乳酸杆菌属产生粪臭素的首次报道。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/242757/a54bb2cf7d52/aem00227-0238-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/242757/a54bb2cf7d52/aem00227-0238-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a5a/242757/a54bb2cf7d52/aem00227-0238-a.jpg

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