Westman S
Biochem J. 1968 Jan;106(2):543-7. doi: 10.1042/bj1060543.
The insulin-degrading activity of liver supernatants and epididymal adipose-tissue homogenates from genetically obese-hyperglycaemic mice (ob ob) and their lean litter mates was studied by measurement of radioactive trichloroacetic acid-soluble degradation products of the insulin molecule. Optimum assay conditions for the decomposition of the hormone were devised. The properties of the degrading activity suggested the presence of enzymic insulin destruction in both the liver and epididymal adipose tissue. There was no difference in insulin degradation in liver samples from obese and lean mice when the results were related to the protein content of the supernatants. The epididymal adipose-tissue homogenates from obese mice displayed about eightfold higher degrading activity per unit of protein than did homogenates from lean animals. The physiological significance of this finding is discussed in the light of the increased fat depots, hyperphagia, raised serum insulin concentrations and increased insulin tolerance previously recorded in this strain of mice.
通过测量胰岛素分子的放射性三氯乙酸可溶性降解产物，研究了遗传性肥胖高血糖小鼠（ob ob）及其瘦同窝小鼠肝脏上清液和附睾脂肪组织匀浆的胰岛素降解活性。设计了激素分解的最佳测定条件。降解活性的特性表明肝脏和附睾脂肪组织中存在胰岛素的酶促破坏。当结果与上清液的蛋白质含量相关时，肥胖和瘦小鼠肝脏样品中的胰岛素降解没有差异。肥胖小鼠的附睾脂肪组织匀浆每单位蛋白质的降解活性比瘦动物的匀浆高约八倍。根据该品系小鼠先前记录的脂肪储存增加、食欲亢进、血清胰岛素浓度升高和胰岛素耐受性增加，讨论了这一发现的生理意义。
