Leleu J B, Fournet B, Morilhat J P, Bonaly R, Montreuil J
Biochimie. 1977;59(8-9):687-92. doi: 10.1016/s0300-9084(77)80247-2.
The treatment with ethylenediamine of Torulopsis candida yeast cell walls has permitted to isolate a mannoprotein. It has been possible to collect this glycoprotein in a pure state after separation of the different hydrosoluble products by gel filtrations and paper electrophoresis. This compound contains 62 per cent of mannose, 3 per cent of N-acetylglucosamine and 31 per cent of amino acids. The mannose units are attached by 1 leads to 2 linkage, short oligosaccharides are bound to serine of the peptide chain by O-glycosidic linkages while polysaccharides are attached to asparagine of this peptide chain by a N glycosidic linkages probably through the intermediate of chitobiose.
用乙二胺处理假丝酵母酵母细胞壁后，已能够分离出一种甘露糖蛋白。通过凝胶过滤和纸电泳分离不同的水溶性产物后，有可能以纯态收集这种糖蛋白。该化合物含有62%的甘露糖、3%的N-乙酰葡糖胺和31%的氨基酸。甘露糖单元通过1→2连接相连，短寡糖通过O-糖苷键与肽链的丝氨酸相连，而多糖可能通过壳二糖中间体通过N-糖苷键与该肽链的天冬酰胺相连。
