Spears J W, Bush L P, Ely D G
J Dairy Sci. 1977 Dec;60(12):1889-93. doi: 10.3168/jds.S0022-0302(77)84120-9.
Twenty-four hours in vitro incubations were used to study the effect of nitrate and molybdenum on sulfur utilization by rumen microorganisms. Sulfur was added as sodium sulfate or sulfide at .1, .2, .3, or .4% of the substrate dry matter. Cellulose digestion was an indicator of microbial growth. The addition of .1 to .4% sulfate or sulfide sulfur increased cellulose digestion over the conrol, the two sulfur sources being equal in promoting cellulose digestion. No differences in cellulose digestion were found between .1 and .4% added sulfur. However, the addition of .4 of .8% nitrate-nitrogen depressed cellulose digestion and increased the requirement for both sulfate and sulfide. Depression was greater with .8% nitrate-nitrogen. In the presence of nitrate, sulfide was superior to sulfate in promoting cellulose digestion. When 4 or 8 ppm molybdenum were added to the incubations, increasing concentrations of both sulfate and sulfide were required to obtain maximum cellulose digestion. Molybdenum additions increased both the sulfate and sulfide requirement for maximum cellulose digestion.
进行24小时的体外培养，以研究硝酸盐和钼对瘤胃微生物利用硫的影响。硫以硫酸钠或硫化物的形式添加，添加量为底物干物质的0.1%、0.2%、0.3%或0.4%。纤维素消化是微生物生长的一个指标。添加0.1%至0.4%的硫酸根或硫离子硫，与对照相比，纤维素消化增加，两种硫源在促进纤维素消化方面效果相同。添加0.1%和0.4%硫时，纤维素消化没有差异。然而，添加0.
