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6-甲基嘌呤对罗氏白绢病菌菌核形态发生的抑制作用及其被腺苷逆转的现象

6-Methylpurine-induced inhibition of sclerotia morphogenesis in Sclerotium rolfsii and its reversal by adenosine.

作者信息

Igwegbe E C, King V, Salary J

出版信息

Mycopathologia. 1977 Dec 31;62(3):153-9. doi: 10.1007/BF00444108.

DOI:10.1007/BF00444108
PMID:563978
Abstract

In liquid synthetic medium inoculated with Sclerotium rolfsii (SR), addition of 6-methylpurine (MP, 50microgram/ml) immediately after inoculation led to approximately 100% reduction in sclerotia production. Adenosine, and to a lesser extent guanosine, each at final concentration of 100microgram/ml significantly reduced inhibition of sclerotia formation by SR in presence of 50microgram/ml MP. Uridine and cytidine each at 100microgram/ml had no such effect. The inhibition of sclerotia morphogenesis could be prevented by addition of 800microgram/ml of adenosine together with 50microgram/ml MP. Reversal by adenosine of MP-induced inhibition of sclerotia development was concentration dependent.

摘要

在接种齐整小核菌(SR)的液体合成培养基中,接种后立即添加6-甲基嘌呤(MP,50微克/毫升)可使菌核产量降低约100%。腺苷以及程度稍低的鸟苷,各自终浓度为100微克/毫升时,在存在50微克/毫升MP的情况下可显著减轻SR对菌核形成的抑制作用。尿苷和胞苷各自浓度为100微克/毫升时则无此效果。添加800微克/毫升腺苷与50微克/毫升MP一起可防止对菌核形态发生的抑制。腺苷对MP诱导的菌核发育抑制的逆转作用呈浓度依赖性。

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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Control of the synthesis of T4 phage deoxynucleotide kinase messenger ribonucleic acid in vivo.T4噬菌体脱氧核苷酸激酶信使核糖核酸在体内合成的调控
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Effect of actinomycin D and chloramphenicol on the induction of the malate decarboxylase system in Lactobacillus thermobacterium 5 (CNRZ) 313.
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