用蔗糖间隙法在兔颈上神经节记录的静息电位和动作电位。

Resting and action potentials recorded by the sucrose-gap method in the superior cervical ganglion of the rabbit.

Kosterlitz H W, Lees G M, Wallis D I

J Physiol. 1968 Mar;195(1):39-53. doi: 10.1113/jphysiol.1968.sp008445.

Abstract

Evoked ganglionic action potentials and drug-induced changes in resting potential were recorded by the sucrose-gap method. The height of the action potential was 10-20 mV.2. Since the amplitude of the P wave is inversely proportional to the external potassium concentration between 0.5 and 12 mM, the P wave is probably caused by a phase of increased permeability to K(+).3. In the absence of an anticholinesterase, the depolarizing actions of choline and acetylcholine on the ganglion are almost equal, whereas acetylcholine is 2-3 times more potent than choline on the fibres of the internal carotid nerve. In the presence of eserine, acetylcholine induces a depolarization of the ganglion of up to 14 mV, while increase in K(+) causes a depolarization of up to 55 mV.4. Although the post-ganglionic fibres in the distal pole of the ganglion are depolarized by drugs, for example, acetylcholine, they contribute little to drug-induced potential changes and probably little to evoked action potentials.5. The hyperpolarization which occurs after exposure of the ganglion to acetylcholine is not due to a phase of increased permeability to K(+).

摘要

用蔗糖间隙法记录诱发的神经节动作电位和药物引起的静息电位变化。动作电位的高度为10 - 20毫伏。
由于在0.5至12毫摩尔之间P波的振幅与细胞外钾浓度成反比，所以P波可能是由钾离子通透性增加的阶段引起的。
在没有抗胆碱酯酶的情况下，胆碱和乙酰胆碱对神经节的去极化作用几乎相等，而乙酰胆碱对内颈动脉神经纤维的作用比胆碱强2 - 3倍。在有依色林存在时，乙酰胆碱可使神经节去极化达14毫伏，而细胞外钾离子浓度升高可使去极化达55毫伏。
虽然神经节远端的节后纤维会被药物（如乙酰胆碱）去极化，但它们对药物诱发的电位变化贡献很小，对诱发的动作电位可能也贡献不大。
神经节暴露于乙酰胆碱后出现的超极化并非由于钾离子通透性增加的阶段所致。