嘌呤霉素和放线菌素D对体外持续性腮腺炎病毒感染的影响。
Effect of puromycin and actinomycin D on a persistent mumps virus infection in vitro.
作者信息
Northrop R L
出版信息
J Virol. 1969 Aug;4(2):133-40. doi: 10.1128/JVI.4.2.133-140.1969.
Abstract
Puromycin and actinomycin D were used to treat a line of human conjunctiva cells persistently infected with mumps virus (C-M cells) in order to determine where virus synthesis is inhibited. Although 90% of the cells in C-M cultures are infected, little or no infectious virus is produced by most cells in a growing culture. Adding puromycin to inhibit protein synthesis resulted in the production of infectious virus. Thus, all the viral proteins needed for virus completion were made in the growing cells. When actinomycin D was added to growing cells, infectious virus was again produced. Since mumps virus synthesis is actinomycin D-insensitive, this suggested a host control of the virus. Interferon was not detected. The possible mechanisms of host control are discussed.
摘要
使用嘌呤霉素和放线菌素D处理持续感染腮腺炎病毒的人结膜细胞系（C-M细胞），以确定病毒合成受抑制的部位。尽管C-M培养物中90%的细胞被感染，但在生长的培养物中，大多数细胞很少或不产生有感染性的病毒。添加嘌呤霉素抑制蛋白质合成导致产生有感染性的病毒。因此，病毒完成所需的所有病毒蛋白都是在生长的细胞中产生的。当向生长的细胞中添加放线菌素D时，再次产生了有感染性的病毒。由于腮腺炎病毒的合成对放线菌素D不敏感，这表明存在宿主对病毒的控制。未检测到干扰素。讨论了宿主控制的可能机制。
相似文献
1
Effect of puromycin and actinomycin D on a persistent mumps virus infection in vitro.嘌呤霉素和放线菌素D对体外持续性腮腺炎病毒感染的影响。
J Virol. 1969 Aug;4(2):133-40. doi: 10.1128/JVI.4.2.133-140.1969.
2
In vitro analysis of experimental avian viral myocarditis.实验性禽病毒性心肌炎的体外分析
J Infect Dis. 1975 Aug;132(2):125-32. doi: 10.1093/infdis/132.2.125.
3
Antibody production in vitro. II. Effects of actinomycin D and puromycin on the secondary response to sheep erythrocytes.体外抗体产生。II. 放线菌素D和嘌呤霉素对绵羊红细胞二次反应的影响。
J Exp Med. 1968 Apr 1;127(4):675-91. doi: 10.1084/jem.127.4.675.
4
Effects of actinomycin D, cycloheximide and puromycin on thyroid stimulation.放线菌素D、放线菌酮和嘌呤霉素对甲状腺刺激的影响。
Endocrinology. 1968 Nov;83(5):1132-9. doi: 10.1210/endo-83-5-1132.
5
Synthesis of macromolecules in epithelial cells of the cultured amphibian lens. II. Effects of puromycin and actinomycin D on protein synthesis.培养的两栖类晶状体上皮细胞中大分子的合成。II. 嘌呤霉素和放线菌素D对蛋白质合成的影响。
Exp Cell Res. 1968 May;50(2):454-9. doi: 10.1016/0014-4827(68)90463-1.
6
Effects of actinomycin D and puromycin on RNA and protein synthesis in Oncopeltus fasciatus after juvenile hormone treatment.放线菌素D和嘌呤霉素对经保幼激素处理的烟草天蛾RNA和蛋白质合成的影响。
Comp Biochem Physiol B. 1972 Apr 15;41(4):771-6. doi: 10.1016/0305-0491(72)90089-2.
7
Inhibition of protein synthesis in Newcastle disease virus infected L cells.
Proc Soc Exp Biol Med. 1971 Sep;137(4):1405-10. doi: 10.3181/00379727-137-35799.
8
Differential effect of actinomycin D on amino acid incorporation by microsomal components of the housefly Musca domestica.放线菌素D对家蝇（Musca domestica）微粒体成分氨基酸掺入的差异效应。
Exp Cell Res. 1971 Nov;69(1):81-91. doi: 10.1016/0014-4827(71)90313-2.
9
Effect of puromycin and actinomycin D on glucose-stimulated protein and RNA labeling, in vitro, in rat testis.
Biochim Biophys Acta. 1972 Mar 24;262(3):336-43. doi: 10.1016/0005-2787(72)90271-7.
10
Effect of ultraviolet irradiation and actinomycin D on polyoma virus replication in mouse embryo cell cultures.紫外线照射和放线菌素D对小鼠胚胎细胞培养中多瘤病毒复制的影响。
J Virol. 1969 Jun;3(6):570-7. doi: 10.1128/JVI.3.6.570-577.1969.
引用本文的文献
1
A protein interaction map identifies existing drugs targeting SARS-CoV-2.蛋白质相互作用图谱鉴定出针对 SARS-CoV-2 的现有药物。
BMC Pharmacol Toxicol. 2020 Sep 3;21(1):65. doi: 10.1186/s40360-020-00444-z.
2
Membrane binding of input arbovirus ribonucleic acid: effect of interferon or cycloheximide.输入虫媒病毒核糖核酸的膜结合：干扰素或环己酰亚胺的作用
J Virol. 1970 Aug;6(2):169-75. doi: 10.1128/JVI.6.2.169-175.1970.
3
Mumps virus replication in chick embryo lung cells: properties of ribonucleic acids in virions and infected cells.腮腺炎病毒在鸡胚肺细胞中的复制：病毒粒子及受感染细胞中核糖核酸的特性
J Virol. 1971 Aug;8(2):161-73. doi: 10.1128/JVI.8.2.161-173.1971.
4
Latency of human measles virus in hamster cells.人麻疹病毒在仓鼠细胞中的潜伏期。
J Virol. 1972 Nov;10(5):995-1001. doi: 10.1128/JVI.10.5.995-1001.1972.
5
Paramyxovirus-avian cell relationship: discrepant impact of 6-azauridine on virus production by susceptible and less susceptible cells.副粘病毒与禽类细胞的关系：6-氮杂尿苷对易感细胞和较不易感细胞产生病毒的不同影响。
Infect Immun. 1975 May;11(5):915-8. doi: 10.1128/iai.11.5.915-918.1975.
6
Persistent infection of tissue culture cells by RNA viruses.RNA病毒对组织培养细胞的持续感染。
Med Microbiol Immunol. 1976 Jun 1;162(2):89-119. doi: 10.1007/BF02121320.
7
Clonal analysis of mammalian cell cultures persistently infected with Japanese encephalitis virus.
J Virol. 1979 Sep;31(3):816-22. doi: 10.1128/JVI.31.3.816-822.1979.
本文引用的文献
1
PERSISTENT INFECTION OF HUMAN CONJUNCTIVA CELL CULTURES BY MYXOVIRUS PARAINFLUENZA 3.副流感病毒3对人结膜细胞培养物的持续性感染
Can J Microbiol. 1965 Jun;11:513-21. doi: 10.1139/m65-068.
2
BINDING OF TRANSFER RIBONUCLEIC ACID TO RIBOSOMES ENGAGED IN PROTEIN SYNTHESIS: NUMBER AND PROPERTIES OF RIBOSOMAL BINDING SITES.参与蛋白质合成的核糖体与转移核糖核酸的结合：核糖体结合位点的数量及特性
J Mol Biol. 1965 Jan;11:35-53. doi: 10.1016/s0022-2836(65)80169-3.
3
EFFECT OF ACTINOMYCIN D AND PUROMYCIN DIHYDROCHLORIDE ON ACTION OF INTERFERON.放线菌素D和盐酸嘌呤霉素对干扰素作用的影响。
Virology. 1964 Dec;24:586-8. doi: 10.1016/0042-6822(64)90211-9.
4
MULTIPLICATION OF A MYXOVIRUS (SV5) WITH MINIMAL CYTOPATHIC EFFECTS AND WITHOUT INTERFERENCE.一种具有最小细胞病变效应且无干扰的黏液病毒（SV5）的增殖
Virology. 1964 Jun;23:224-33. doi: 10.1016/0042-6822(64)90286-7.
5
The role of protein synthesis in the eclipse period of newcastle disease virus multiplication in HeLa cells as studied with puromycin.用嘌呤霉素研究蛋白质合成在新城疫病毒在HeLa细胞中增殖的隐蔽期的作用。
Proc Natl Acad Sci U S A. 1962 Aug;48(8):1358-66. doi: 10.1073/pnas.48.8.1358.
6
A carrier state of mumps virus in human conjunctiva cells. II. Observations on intercellular transfer of virus and virus release.人类结膜细胞中腮腺炎病毒的携带状态。II. 病毒细胞间转移及病毒释放的观察
J Exp Med. 1962 Nov 1;116(5):751-8. doi: 10.1084/jem.116.5.751.
7
A carrier state of mumps virus in human conjunctiva cells. I. General characteristics.人类结膜细胞中腮腺炎病毒的携带状态。I. 一般特征。
J Exp Med. 1962 Nov 1;116(5):739-50. doi: 10.1084/jem.116.5.739.
8
Growth of Newcastle disease virus in a HVJ carrier culture of HeLa cells.新城疫病毒在HeLa细胞的HVJ载体培养物中的生长情况。
Virology. 1966 Jun;29(2):255-63. doi: 10.1016/0042-6822(66)90032-8.
9
Persistent, noncytocidal viral infection: nonsynchrony of viral and cellular multiplication.持续性非细胞病变性病毒感染：病毒与细胞增殖不同步。
J Bacteriol. 1966 Oct;92(4):983-9. doi: 10.1128/jb.92.4.983-989.1966.
10
On the role of the response of the cell membrane in determining virus virulence. Contrasting effects of the parainfluenza virus SV5 in two cell types.关于细胞膜反应在决定病毒毒力中的作用。副流感病毒SV5在两种细胞类型中的不同作用。
J Exp Med. 1966 Sep 1;124(3):501-20. doi: 10.1084/jem.124.3.501.
Zotero 插件